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Insights in Enzyme Research
ISSN: 2573-4466
E u r o S c i C o n C o n g r e s s o n
Enzymology and
Molecular Biology
A u g u s t 1 3 - 1 4 , 2 0 1 8
P a r i s , F r a n c e
Enzymology 2018
All aerobic organisms generate reactive oxygen species, which are highly
reactive against most of the molecules present in the cell. Organisms
produce antioxidant molecules in order to eliminate the harmful effects
of reactive oxygen species. Antioxidant molecules are diverse type of
compounds, such as enzymes, minerals, vitamins, carotenoids, polyphenols,
etc. Biophenols, which are secondary metabolites, also have a significant
role as antioxidant molecules. Olive leaf is a valuable natural source regarding
biophenolic compounds. These compounds include oleuropein, verbascoside,
rutin, apigenin-7-glucoside, hydroxytyrosol, tyrosol, caffeic acid, p-coumaric
acid, vanillic acid, vanillin, etc. Many researches have been made for the
extraction of biophenolics from olive leaf. They are commonly extracted by
using organic solvents, mainly ethanol and methanol and/or with water.
Green solvents gain increasing attention in many fields of research due to
the increasing conscious of protection of the environment. Reports show
that green solvents are good candidates to replace organic solvents in many
biotechnological processes and are under extensive research for the ones that
it is not yet. In this study, we investigated the effect of different types of deep
eutectic solvents as green solvents on the extraction of biophenolics from olive
leaf and compared the extraction yield and the type of extracted biophenols
with conventional solvents. It was found that most of deep eutectic solvents
tested provided promising results also depending on the extraction conditions.
Biography
Ayse Ezgi Unlu graduated from Ankara University, Faculty of
Engineering, Department of Chemical Engineering in 2002.
She completed her master degree in 2005 at Ankara University
in Turkey. The synthesis of Naproxen, a member of NSAIDs,
was the subject of the master thesis using commercial lipase
subjected to various pre-treatment strategies that enhanced the
activity. Investigation of different parameters on the production
of lipase by Candida rugosa and also proteomic analysis of
the isoenzymes was another subject of interest. Ayse Ezgi
Unlu completed her Ph.D. in 2012 at Ankara University in
Turkey. Two important antioxidant enzymes, catalase and
superoxide dismutase production by Rhodotorula glutinis was
studied comprehensively during PhD thesis. She received a
postdoctoral grant from TUBITAK, with a project about the
synthesis of flavonoid polymers using green solvents, at the
Institute of Technical Biocatalysis, Technical University of
Hamburg, Harburg in Germany, between 2014-2015. She
is currently working at Biotechnological Research Group in
the Department of Chemical Engineering, Ankara University.
The research area includes enzymes, enzymatic reactions,
fermentation, protein synthesis, proteomics, experimental
design, enzymatic biopolymers and green solvents.
The extraction of biophenolics from olive leaf using green solvents
Ayse Ezgi Unlu, Arıkaya A and Takac S
Ankara University, Ankara, Turkey
Ayse Ezgi Unlu et al., Insights Enzyme Res 2018, Volume 2
DOI: 10.21767/2573-4466-C1-002