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Insights in Enzyme Research
ISSN: 2573-4466
E u r o S c i C o n C o n g r e s s o n
Enzymology and
Molecular Biology
A u g u s t 1 3 - 1 4 , 2 0 1 8
P a r i s , F r a n c e
Enzymology 2018
I
schemic stroke recovery is associated with neural stem cells (NSCs)
development and neurovascular unit reconstruction. Exosomes, as important
intercellular players in cellular communication, mediate neuro-restorative
events, however, their effects / mechanisms in the injured brain are unknown.
The objective of this study is to determine the effect of human NSCs-derived
exosomes on the potential abilities of cells, and whether human NSCs-derived
exosomes can repair the functions of ischemic stroke rats. This study finds
that IFN-γ stimulating managed the abilities of human NSCs-derived exosomes
(hNSCs-Exo), including alleviated the level of oxidative stress of NSCs following
H2O2 stimulating, augmented the NSC survival, and promoted the neuronal
differentiation of NSCs. Furthermore, in rats ischemic stroke model, IFN-γ-
hNSCs-Exo further facilitated the neurological functional recovery (such as
modified Neurological Severity Score, Rotarod test, etc.) compared to hNSCs-
Exo group, enhanced nerve cell survival and promoted neovascularization
(microvessel density, MVD). Importantly, exosomes can be internalized or
endocytosed by cells, after labeled with PKH67, it showed that exosomes
migrated to the infarct regions together with cells, as interestingly, many
exosomes entry into the nucleus. Next generation sequencing (NGS) revealed
significant enrichment of hsa-miR-206 and hsa-miR-133a-3p in IFN-γ-hNSCs
exosomes compared with hNSCs exosomes. The mechanisms or effects of
exosomes were to deliver specific exosomal microRNAs to cells for increasing
cell survival and proliferation. In summary, hNSCs-derived exosomes possess
the ability of neural regeneration and modulate neurological functional
recovery, and play more positive roles by stimulating with IFN-γ (IFN-γ-hNSCs
Exo). Exosomes provide a novel and promising strategy in modulating disease
treatment and tissue regeneration, avoiding the risk of teratomas associated
with cells.
Biography
Guilong Zhang is currently pursuing his PhD at the School of
Medicine, Southeast University, Nanjing, China. His major is
Neurosurgery, and will graduate at 2018/2019. His research
field is Stem Cells Therapy and Clinical Translational Research.
He has published about 10 papers in molecular and biomedical
journals.
longshe05@sina.comExosomes derived from human neural stem cells mediate
cellular stress ability and promote neurological function
recovery of cerebral ischemic rats
Guilong Zhang, Lukui Chen, Hong Wang, Bingqian Li, Wanghao Chen,
Yongbo Yu and ZhiHan Zhu
Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China
Guilong Zhang et al., Insights Enzyme Res 2018, Volume 2
DOI: 10.21767/2573-4466-C1-002
Figure: 1
The role of IFN-γ on NSCs
Figure: 2
The identity of exosomes
Figure: 3
The GO categories of NGS