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Insights in Enzyme Research
ISSN: 2573-4466
E u r o S c i C o n C o n g r e s s o n
Enzymology and
Molecular Biology
A u g u s t 1 3 - 1 4 , 2 0 1 8
P a r i s , F r a n c e
Enzymology 2018
S
cientists have been trying to develop biosensors with great efforts for the
wide fields of application including medical diagnostics, environmental
monitoring, and food analysis for many years. The development of biosensors
has become increasingly important in molecular diagnostics. They need
new methods continuously to get higher sensitivity, lower cost, and better
reproducibility. Because of their high sensitivity property, fluorescence and
chemiluminescence are widely used in clinical diagnostic laboratories.
However, the high instrument cost is a major disadvantage for these
methods. Compared to optical detection methods, electrochemical methods
are much simpler and not expensive. Among the various electrochemistry-
based biosensors developed so far, the amperometric glucose sensing
approach has attracted a great deal of attention. Conducting polymers,
CPs, are used as suitable matrices supplying common properties for
biomolecule immobilization. Their compatibility with biological molecules,
easy preparation, high reproducibility and electrochemical properties make
them fascinating in biosensor design. The dithieno [3, 2-b:2',3'-d] pyrrole (DTP)
based conjugated organic materials have been precursor materials because
their chemical structure with strong electron donating ability promises easily
functionalization for polymers. Different unit substitution from pyrrole unit
in excellent molecular DTP structures has been bringing a breath of fresh
air to the field of conjugated polymer since thiophene-pyrrole-thiophene co-
monomer structure has a fused ring system and good planar structure, which
make an extended conjugated polymer during electropolymerization step. For
that reason, a remarkable amount of research effort has been committed to
synthesize and create applications of novel DTP based conjugated polymers.
This presentation reports amperometric biosensors constructed by using DTP
type conducting polymers and important enzymes in food technology which
are glucose oxidase, alcohol oxidase, xantine oxidase. The biosensors showed
outstanding analytical properties of high sensitivity, selectivity, and reliability
being applied to food samples.
Biography
Huseyin Bekir Yildiz has completed his PhD in Chemistry
from the Middle East Technical University and Postdoctoral
studies from Institute of Chemistry at the Hebrew University
of Jerusalem, Department of Chemistry at University of
California Berkeley, Center for Molecular Protein Science
at Lund University and Institute for Applied Biosciences at
Technical University of Applied Sciences Wildau. He is affiliated
to Department of Metallurgical and Materials Engineering, KTO
Karatay University, where he is currently working as Professor.
He is also the Director of Graduate School of Engineering and
Natural Sciences at the same university. He has publishedmore
than 50 papers in reputed journals and has been serving as an
Editorial Board Member of repute.
huseyinbekir.yildiz@karatay.edu.trMaking novel enzymatic biosensors by using DTP type conducting
polymers
Huseyin Bekir Yildiz
1
, Hacer Azak
2
, Emre Cevik
3
, Buket Carbas
2
and
Mehmet Senel
4
1
KTO Karatay University, Turkey
2
Karamanoglu Mehmetbey University, Turkey
3
IRMC-Imam Abdulrahman Bin Faisal University, Saudi Arabia
4
SANKARA Brain & Biotechnology Research Center, Istanbul Biotechnology Inc., Turkey
Huseyin Bekir Yildiz et al., Insights Enzyme Res 2018, Volume 2
DOI: 10.21767/2573-4466-C1-002