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Insights in Enzyme Research
ISSN: 2573-4466
E u r o S c i C o n C o n g r e s s o n
Enzymology and
Molecular Biology
A u g u s t 1 3 - 1 4 , 2 0 1 8
P a r i s , F r a n c e
Enzymology 2018
A
mong the plant derived product, azadirachtin, a neem-based insecticide,
is exceptional in having a broad range of bioactivity including toxicity,
growth, development and reproductions effects, repellency and antifeedancy.
If considerable progress on the physiological and biological activities and
agricultural application of azadirachtin had been achieved, its exact mechanism
of action remains uncertain. In this study, we evaluated lethal and sublethal
effects of azadirachtin on digestive enzymes of
Drosophila melanogaster
as
biological model. The bio-insecticide was applied topically on early third instar
larvae (L3) at two doses, DL
25
(0, 28 µg) and DL
50
(0, 67 µg). Results showed
a clear disruption of digestive enzymes activities responsible for the broken
down of dietary components before its absorption by the intestinal epithelium.
Indeed, an inhibition of α-amylase, chitinase and protease activities and an
increase of lipasic activity were noted. These results may reflect interference
of azadirachtin with regulation of metabolism, and provide some evidence of
a long term antifeedancy and delayed effects through developmental stage
which may reinforce the insecticidal activity of this bioinsecticide.
Biography
Maroua Ferdenache was awarded a Master's Degree in Biology
by the University of Annaba (Algeria), graduated with first class
honors and the winner of the doctoral competition. Last year,
she got a scholarship to Paris-Saclay University. Now she
is a first year PhD student at the French National Center for
Scientific Research (CNRS) in Paris (France). She participated in
4 conferences and till now she have 2 papers in reputed journals
and preparing another one.
maroua.ferdenache@gmail.comPreimaginal exposure to azadirachtin affects digestive enzymes
in adults of
Drosophila melanogaster
(Diptera: Drosophilidae)
Maroua Ferdenache, Samira Kilani-Morakchi and
Radia
Bezzar-Bendjazia
Badji Mokhtar University of Annaba, Annaba, Algeria
Maroua Ferdenache et al., Insights Enzyme Res 2018, Volume 2
DOI: 10.21767/2573-4466-C1-002