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Insights in Enzyme Research
ISSN: 2573-4466
E u r o S c i C o n C o n g r e s s o n
Enzymology and
Molecular Biology
A u g u s t 1 3 - 1 4 , 2 0 1 8
P a r i s , F r a n c e
Enzymology 2018
Ethyl lactate is a commonly used biodegradable solvent and it is widely used
in food additives, pharmaceutical preparations, and also in fragrances. It
is naturally found in alcoholic beverages, various foods, such as cabbage,
vinegar, butter, chicken and some fruits. In the literature, searches on ethyl
lactate synthesis by the esterification reaction of lactic acid are mainly focused
on i) the enhancement of the product yield by removing water employing
various procedures ii) the facilitation of down-stream processing by using
heterogeneous catalysis, and iii) the environmentally friendly processes by
using enzymes. In this study, we investigated the synthesis of ethyl lactate by
esterification reaction of lactic acid using lipase enzyme in a choline chloride-
based deep eutectic solvent as green reaction medium. The synthesis of ethyl
lactate was carried out in a batch system under different reaction conditions.
According to the results, the initial reaction rate increased with the increase in
initial lactic acid concentration at constant ethanol concentration. The molar
ratio of the substrates strongly affected the rate of this reversible reaction.
The temperature of the synthesis was found to have a significant effect on the
initial reaction rate. Bell-shaped curve was obtained for the initial reaction rate
as the temperature increased. High agitation rates increased the reaction rate
by decreasing the mass transfer limitation in the medium. The overall results
showed that deep eutectic solvent was successfully used in the esterification
reaction of lactic acid.
Biography
Ayse Ezgi Unlu graduated from Ankara University, Faculty of
Engineering, Department of Chemical Engineering in 2002.
She completed her master degree in 2005 at Ankara University
in Turkey. The synthesis of Naproxen, a member of NSAIDs,
was the subject of the master thesis using commercial lipase
subjected to various pre-treatment strategies that enhanced the
activity. Investigation of different parameters on the production
of lipase by Candida rugosa and also proteomic analysis of
the isoenzymes was another subject of interest. Ayse Ezgi
Unlu completed her Ph.D. in 2012 at Ankara University in
Turkey. Two important antioxidant enzymes, catalase and
superoxide dismutase production by Rhodotorula glutinis was
studied comprehensively during PhD thesis. She received a
postdoctoral grant from TUBITAK, with a project about the
synthesis of flavonoid polymers using green solvents, at the
Institute of Technical Biocatalysis, Technical University of
Hamburg, Harburg in Germany, between 2014-2015. She
is currently working at Biotechnological Research Group in
the Department of Chemical Engineering, Ankara University.
The research area includes enzymes, enzymatic reactions,
fermentation, protein synthesis, proteomics, experimental
design, enzymatic biopolymers and green solvents.
Enzymatic ethyl lactate synthesis in a green reaction medium
Ayse Ezgi Unlu, Arıkaya A and Takac S
Ankara University, Ankara, Turkey
Ayse Ezgi Unlu et al., Insights Enzyme Res 2018, Volume 2
DOI: 10.21767/2573-4466-C1-002