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Environmental Science & Technology 2018

Journal of Environmental Research

Page 56

March 29-31, 2018

Vienna, Austria

4

th

Edition of International Conference on

Environmental Science

& Technology 2018

S

aline soils are widely distributed across the world. The

application of beneficial microorganisms is a novel technology

to reduce salt stress and improve plant productivity. Phosphate-

solubilizing microorganisms (PSM) are a group of plant growth-

promoting rhizobacteria, which can dissolve insoluble phosphates

and maintain the nutrient status of salt soil. To identify efficient

PSMwith multiple activities that promote the plants to grow in the

salinesoil,weconductedasurveyofPSMnaturallycolonizingsaline

soil of the Yellow River, China. A total of 42 PSMs were isolated,

the majority clustered together as

Bacillus

spp., one

Providencia

rettgeri

sp. strain was confirmed as PSM for the first time. The

capacity of dissolving inorganic phosphorus analysis was carried

out by agar plate and liquid culture. Ten isolates classified as the

best solubilizers with solubilization rates greater than 200 mg/L.

In contrast, the PSM isolates were less effective when solubilizing

ferric phosphate (FePO

4

) or aluminum phosphate (AlPO

4

). The

organic acid types and content were tested by high performance

liquid chromatography (HPLC) method. Eight different organic

acids (oxalic acid, gluconic acid, lactic acid, succinic acid,

formic acid, citric acid and malic acid and propionic acid) with

different content were detected in the culture filtrates. Isolates

were also checked for indole-3-acetic acid (IAA), siderophore

and exopolysaccharide. All of the isolates could secrete IAA

within the range 2.7~31.8 mg L/1 and exopolysaccharide within

the range 74.3~225.7 mg L/1. There were 12 (28.6% of the total

strains) siderophore-producing strains with the siderophore unit of

1.9~42.1%. This initial study on PSM isolates distributed in saline

soil showed that some isolates show promise for potential use as

bio-inoculants for promoting plant growth in saline environments.

Biography

Huanhuan Jiang is pursuing her PhD degree in Environmental Engineering

at Harbin Industrial University and will complete her PhD in June 2018. Her

research interests cover the theory and application of resources recovery

from waste (water)/bio solids, organic wastes (excess sludge, waste bio-

mass, etc.) cascade utilization to recover bio resources and/or bioenergy,

soil restoration and soil fertility by Microbial e.g. She has participated in the

project of China Agriculture Research System, The National Natural Science

Foundation of China etc. At the period of her graduate study, her four papers

were under review.

767360114@qq.com

Isolation and characterization of phosphate-solubilizing

microorganisms with bio control potential from salt-affected soil

Huanhuan Jiang

1

, Peishi Qi

1

, Shanlin Yu

2

, Tong Wang

2

, Mian Wang

2

, Mingna

Chen

2

, Na Chen

2

, Xiaoyuan Chi

2

and

Lijuan Pan

2

1

Harbin Institute of Technology, China

2

Shandong Peanut Research Institute, China

Huanhuan Jiang et al., J Environ Res, Volume 2