9 / 13
Advanced Stem Cell 2018
Journal of Stem Cell Biology and Transplantation
ISSN: 2575-7725
Page 44
December 03-04, 2018
Valencia, Spain
15
th
Edition of EuroSciCon Conference on
Advanced Stem Cell &
Regenerative Medicine
D
ental pulp, periodontal ligament, dental follicle, apical
papilla, oral mucosa, periodontal granulation tissue, palatal
tissue is considered easily accessible and important sources of
mesenchymal stem cell (MSCs). In the current study, we isolated
and assessed human dental germs and palatal connective tissue
stem cells. MSCs were obtained from dental germs of wisdom
teeth and palatal tissue samples were used from clinically healthy
patients undergoing intervention due to the absence of space
required for the eruption. After isolation the cells were cultured in
propagationmedium. Cellsmorphology, colony formingefficiency,
population doubling capacities and multilineage differentiation
potentials were investigated. After 1st passage, both cell lines
possessed fibroblast like morphology, the frequency of colony
forming efficiency for dental germs derived mesenchymal stem
cells (dgMSCs) was significantly higher than that of palatal
connective tissue derived mesenchymal stem cells (pMSCs).
Significantly higher population doubling time was recorded for
dgMSCs. The specific antigen makeup of the isolated MSCs
were characterized in the 4th passage using a FACSCanto II flow
cytometer (BD Biosciences, San Jose, CA, USA) and analyzed
using the DIVA program. Both cell lines were positive for CD105,
CD73, CD90, CD44, and CD49f and negative for CD34, CD45, and
HLA-DR, but the levels of expression showed small differences.
MSCs from both cell lines were successfully differentiated
into osteogenic, adipogenic and chondrogenic lineages. Our
preliminary results suggest that isolation, identification and
immuno phenotyping of dgMSCs and pMSCs are feasible and
may represent an easily available source for oral regenerative
therapies.
emoke.pall@usamvcluj.roDental germs and palatal connective tissue stem
cells: tools for oral regenerative therapy
Emoke Pall
1
, Olga Soritau
2
, Alexandra Roman
3
and
Meda Simu
3
1
University of Agricultural Sciences and Veterinary Medicine of Cluj Napoca, Romania
2
The Oncology Institute Prof. Dr. Ion Chiricuţă, Romania
3
Iuliu Hatieganu University of Medicine and Pharmacy, Romania
J Stem Cell Biol Transplant 2018, Volume 2
DOI: 10.21767/2575-7725-C1-003