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Advanced Stem Cell 2018
Journal of Stem Cell Biology and Transplantation
ISSN: 2575-7725
Page 40
December 03-04, 2018
Valencia, Spain
15
th
Edition of EuroSciCon Conference on
Advanced Stem Cell &
Regenerative Medicine
M
ethylprednisolone (MP), a glucocorticoid steroid, has an anti-
inflammatory action and seems to inhibit the formation of
oxygen free radicals produced during lipid peroxidation in a spinal
cord injury (SCI). Currently MP is the standard therapy after acute
SCI on reported neurological improvements. The combination
therapeutic effect of human umbilical cord blood-derived
mesenchymal stem cells (hUCB-MSCs) for transplantation time
(1d, 7d, and 30d) after MP treatment on the axonal regeneration
and on the behavioral improvement in SCI were studied in the rat.
The spinal cord was injured by contusion using a weight-drop
at the level of T9 and MP (30 mg/kg, i.m., 10 min and 4 h) was
acutely administered after injury. hUCB-MSCs were labeled with
GFP and our study performed the efficacy for transplantation
time (1d, 7d, and 30 d) of hUCB-MSCs into the boundary zone of
injured site. Efficacy was determined by histology, anterograde
and retrograde tracing, and behavioral test. We found that
hUCB-MSCs with MP treatment exerted a significant beneficial
effect by neuroprotection and reducing cavity volume. Also the
transplantation of hUCB-MSCs with MP treatment significantly
improved functional recovery. Combined transplantation at
7d after SCI provided significantly greater efficiency than
combined transplantation at 1d and 30d. These results suggest
that transplantation time window of the hUCB-MSCs with MP
treatment give rise to an earlier neuron protection strategy and
effect of cell grafting in SCI. Thus our study may be considered as
a therapeutic modality for SCI.
Biography
Lee So Maeng graduated from the College of Medicine, The Ewha Wom-
en’s University as Medical Doctor with specialties in pathology from the The
Catholic University of Korea (CUK), Republic of South Korea. She obtained
her Postgraduation from the same university and worked on the topic enti-
tled “Sequential changes in aberrant crypt foci and lectin expression in the
early and late stages of DMH-induced colon carcinogenesis in rats”. She is
presently working at the Clinical Research Insitute of the Incheon St. Mary’s
Hospital at the College of Medicine (CUK).
mlsckb1004@gmail.comTherapeutic effects of human mesenchymal
stem cells with methylprednisolone treatment in
rat spinal cord injury
Lee So Maeng
1
, Sang In Park
2
, Ho Yong Jung
2
and
Yong An Chung
2
1
St. Mary’s Hospital – CUK, Republic of South Korea
2
Clinical Research Insitute (CRI), Incheon St. Mary’s Hospital (CUK), Republic of South Korea
Lee So Maeng et al., J Stem Cell Biol Transplant 2018, Volume 2
DOI: 10.21767/2575-7725-C1-003