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Advanced Stem Cell 2018
Journal of Stem Cell Biology and Transplantation
ISSN: 2575-7725
Page 48
December 03-04, 2018
Valencia, Spain
15
th
Edition of EuroSciCon Conference on
Advanced Stem Cell &
Regenerative Medicine
K
eratoconjunctivitis sicca (KCS) or dry eye syndrome, is
predominantly immunomediated and dogs are an excellent
model for understanding this disease due to immunomediated
origin similar to that in humans. The objective is to compare the
expression of immunological markers interleukin (IL), IL-1 and
IL-6, tumor necrosis factor alpha (TNFα), and CD4T lymphocytes,
before and after topical treatment with mesenchymal stem
cells (MSC) in KCS in dogs. Twenty-two dogs, with a bilateral
diagnosis of KCS, were treated topically with 50 μl (1x106 MSC)
in the conjunctival sac in 4 applications at 7 day intervals, and
evaluated monthly for 6 months. For analysis of the expression
of IL-1, IL-6, TNFα, and CD4 markers, two collections were
performed, one before and another at the end of the study, by
fine-needle aspiration of the third eyelid gland and processed by
immunocytochemistry and biopsy of the conjunctival palpebral
and processed by immunohistochemistry. The results obtained
through color density showed that at the pre KCS treatment
moment there was a marked expression of all markers (IL-1, IL-
6, TNFα, and CD4) and after 6 months there was a significant
reduction in the marked area of all markers. These results
demonstrated that these markers could be excellent tools for the
diagnosis and progression of KCS and topical treatment of KCS
with MSC was shown to promote a significant decrease in the
expression of these markers after treatment which in future may
represent another adjuvant therapy option in the treatment of KCS
in dogs and humans.
silviafranco@unoeste.brExpression of immunological markers in the
lacrimal gland and conjunctive palpebral of
dogs with keratoconjunctivitis sicca topically
treated with mesenchymal stem cells
Silvia Franco Andrade
1
, Marcos Rogério Sgrignoli
1
, Danielle Alves Silva
1
, Felipe
Franco Nascimento
1
, Danielle Antonelli Motta
1
, Gisele Alborghetti Nai
2
, Márcia
Guimarães da Silva
3
, Michele Andrade de Barros
4
, Maura Krähembühl Wander-
ley Bittencourt
4
, Heloíse Rangel Dinallo
1
, Bruna Toledo Duran Foglia
1
, Welling-
ton Bott Cabrera
1
and
Elaine Carrion Fares
1
1
University of West of Sao Paulo, Cidade, Brazil
2
University of West of Sao Paulo, Bairro Limoeiro, Brazil
3
Sao Paulo State University, Botucatu, Brazil
4
Regenera, Brazil
J Stem Cell Biol Transplant 2018, Volume 2
DOI: 10.21767/2575-7725-C1-003