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Advanced Stem Cell 2018 Journal of Stem Cell Biology and Transplantation
ISSN: 2575-7725 Page 37
December 03-04, 2018
Valencia, Spain
15th Edition of EuroSciCon Conference on
Advanced Stem Cell &
Regenerative Medicine
T he generation of organoids from human pluripotent stem
cells allows deeper insight into human development, disease
modeling, drug screening and regenerative medicine. Organoids
are in vitro generated 3D structures containing multiple cell types
that are organized similar to an organ, recapitulate some specific
organ function and can mimic the responses of real tissues.
Different human mini lungs (organoids) have been generated
from both human embryonic and induced pluripotent stem cells
(hESCs and hiPSCs respectively). We show here the generation of
2D and 3D mini lungs from two hESCs lines (AND-1 and AND-2)
and have analyzed the effects of vitamin D and two non-calcemic
vitamin D analogs in the expression of DNA damage induced by
the antibiotic bleomycin, an agent widely used to induce lung
fibrosis. The results indicate that bleomycin induced DNA double
strand breaks in both types of structures and that vitamin D
elevated its number. In addition, the two vitamin D analogs tested
were able to reduce the DNA damage observed. The results are
discussed in terms of the role of DNA damage in lung fibrosis and
the potential use of vitamin D analogs in conditions characterized
by DNA damage and senescence such as lung fibrosis.
Biography
Ms. Esmeralda Magro is a third year PhD student in Molecular Biosciences
at the University Autonomous of Madrid. She is conducting a research proj-
ect at the Institute of Health Carlos III, under the supervision of Dr. Alberto
Zambrano. She did her thesis at Dr. Zambrano´s lab. This work was
related to the role of vitamin D on DNA damage expression and cellular se-
nescence in alveolar epithelial cells type II and human fibroblasts and its
potential use as an antifibrogenic agent in Idiopathic Pulmonary Fibrosis
(publication under review). She signs this work as co-first author. Currently,
she is working on her thesis which is focused on the generation of two and
three- dimensional human mini-lungs from human embryonic stem cells to
model chronic respiratory diseases, preferably, the Idiopathic Pulmonary Fi-
brosis (IPF). She is also interested in studying the effect of different drugs
and ligands for nuclear hormone receptors in DNA damage expression and
cellular senescence in the context of lung fibrosis (Magro-Lopez E et al.,
2017, Viruses) and (Magro-Lopez E. et al., 2018, Stem Cell Res Ther).
Generation of mini lungs from human embryonic
stem cells: analysis of the effects of vitamin D
in the expression of DNA damage induced by
bleomycin
Esmeralda Magro López, Alberto Zambrano Duarte and Isabel Liste
Institute of Health Carlos III, Spain
Esmeralda Magro López et al., J Stem Cell Biol Transplant 2018, Volume 2
DOI: 10.21767/2575-7725-C1-003