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Advanced Stem Cell 2018 Journal of Stem Cell Biology and Transplantation
ISSN: 2575-7725 Page 36
December 03-04, 2018
Valencia, Spain
15th Edition of EuroSciCon Conference on
Advanced Stem Cell &
Regenerative Medicine
Human umbilical cord and adipose tissue are among the
richest sources of mesenchymal stemcells (MSCs) with great
promises in the field of regenerative medicine. Regardless of the
intensive investigations of human MSCs from different tissues,
the molecular mechanisms regulating the undifferentiated state
and differentiation abilities are still unclear. The transcription
factors OCT4, NANOG and SOX2 that are crucial for the efficient
maintenance of the fine balance between self-renewal and
differentiation in embryonic stem cells have been suggested
to play a similar role also in mesenchymal stem cells. In the
present work, the expression evaluation of OCT4A, OCT4B and
OCT4B1 splice variants, NANOG and SOX2 were performed in
MSC isolated from umbilical cord (UC), and adipose tissue (AT).
UC-MSC displayed lower population doubling time than AT-MSC,
(30.8±5h, 54.9±5h, respectively). Both cell types expressed the
pluripotent markers OCT4A, NANOG and SOX2. The mRNA levels
of OCT4B and NANOG were significantly higher in AT-MSC than
UC-MSC (p<0.05). In addition, AT-MSC from different patients
showed increased heterogeneity in mRNA levels of all analyzed
genes compared to UC-MSC. In conclusion, higher expression
of NANOG and OCT4B is not associated with better proliferative
potential of AT-MSC. The UC-MSC from different samples
showed lower variation in mRNA levels of OCT4A, OCT4B,
OCT4B1, NANOG and SOX2 than AT-MSC, that make them a more
appropriate candidate for clinical trials.
Biography
E Stoyanova pursued her PhD (2015) from the Institute of Biology and Im-
munology of Reproduction (IBIR) at the Bulgarian Academy of Sciences,
Bulgaria. Currently, she is a Posdoctoral Researcher in the Department of
Molecular biology at the same institute. Her research interests focuses on
reprogramming of human soamtic cells, mesenchymal stem cells derived
from bone marrow, adipose tissue, umbilical cord and cancer cells.
Expression of pluripotency associated genes in
human mesenchymal stem cells derived from
umbilical cord and adipose tissue
E Stoyanova1, S Kestendjieva1, M Kostadinova1, L Dzerov2, A Nikolov2 , T Oresh-
kova1 and M Mourdjeva1
1Institute of Biology and Immunology of Reproduction - BAS, Bulgaria
2University Hospital of Obstetrics and Gynaecology Maichin Dom, Bulgaria
E Stoyanova et al., J Stem Cell Biol Transplant 2018, Volume 2
DOI: 10.21767/2575-7725-C1-003