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Advanced Stem Cell 2018
Journal of Stem Cell Biology and Transplantation
ISSN: 2575-7725
Page 47
December 03-04, 2018
Valencia, Spain
15
th
Edition of EuroSciCon Conference on
Advanced Stem Cell &
Regenerative Medicine
B
one marrow transplantation (BMT) required for the treatment
of hematopoietic diseases encounters challenges like limited
availability of healthy cells, suboptimal homing, graft rejection
etc. and therefore requires atleast 1-10x106 CD34+ stem cells/
kg body weight for higher chances of successful BMT. Dynamic
nature of endothelial cells (ECs) not only allows it to control
the traffic across the organ but also to regulate coagulation,
angiogenesis, blood fluidity etc. which makes them potential
target for therapeutics. As functional regulator EC express
selective molecules to aid in functioning of the underlying tissue.
For instance, vascular cell adhesion molecule-1 (VCAM1) which
has been shown to control homing of infused stem cells and
subsequent engraftment is constitutively expressed on BMECs
only. In an attempt to exploit it as capturing ligand, we have
designed a peptide-polymer based system that can target infused
cells to BM thus enhancing targeted delivery and reducing the
number of stem cells required for successful BMT. The system
constitutes a peptide specific for VCAM1 conjugated to polymers
(like chitosan, PAH, PSS, liposomes etc.) that can encapsulate
cells shielding them from host immune system. To assess the
potential of this system we conjugated it to dextran coated iron
oxide nanoparticles (Dex-IOP) and observed an increase of
nanoparticle delivery to BM by 22%, 46%, 51.5% and 43% for Dex-
IOP and the peptide conjugated to liposome, chitosan and PSS
and PAH respectively. Evaluation of this system to target cells to
BM and its regeneration is being explored and will be discussed in
detail during the conference.
itsraining.swati@gmail.comPeptide-polymer based system for targeting
human stem cells to bone marrow
Swati Gupta and Gurudutta Gangenahalli
INMAS-DRDO, India
J Stem Cell Biol Transplant 2018, Volume 2
DOI: 10.21767/2575-7725-C1-003