14 / 27
A p r i l 2 2 - 2 3 , 2 0 1 9
A t h e n s , G r e e c e
Page 66
Archives of Clinical Microbiology
ISSN: 1989-8436
Virology and Infectious Diseases 2019
EuroSciCon Conference on
Virology and Infectious Diseases
Aim:
Screening of seaweed-associated bacteria capable of producing antimicrobials.
Methods & Results:
Fifteen microbial strains, associated to the brown seaweed Pelvetia canaliculata (Linnaeus)
attached to the rocks of Sonmiani Beach (Karachi, Pakistan), were screened. Crude extract filtrates of CMG S2 strain
grew on Zobell marine agar (ZMA) had the most remarkable antimicrobial activity. Based on its phenotypic aspects
(e.g. Gram-positive, microccoid form), biochemical characteristics (e.g. halotolerance) and genetic analyses, CMG S2
is identified as a putatively new Kocuria marina type strain belonging to the actinobacteria's class and micrococcaceae
family. Thereby, the nucleotide sequence analysis of its full-length 16S ribosomal ribonucleic acid (rRNA) gene
(GenBank accession number EU073966.1) displayed highest identity (i.e. 99%) and score (2630) with K. marina KMM
3905. Phylogenic trees analysis using the neighbor-joining method showed closest evolutionary distance of CMG S2
with KMM 3905 strain and
K carniphila
(DC2201) species. Interestingly, a unique ultraviolet (UV)-bioactive compound
was purified from CMG S2 crude extracts by flash silica gel column and thin-layer chromatography (TLC) techniques. Its
chemical structure was unravelled as 4-[(Z)-2 phenyl ethenyl] benzoic acid (PEBA, later named kocumarin) by nuclear
magnetic resonance (NMR) spectroscopy techniques. Importantly, kocumarin demonstrated prominent and rapid
growth inhibition against all tested fungi and pathogenic bacteria including methicillin-resistant
Staphylococcus aureus
(MRSA), with a minimal fungal inhibitory concentration (MFC) of 15-25 μg/mL and a minimal (bacterial) inhibitory
concentration (MIC) of 10-15 μg/mL.
dr.fmenaa@gmail.comIsolation, purification, structural elucidation
and antimicrobial activities of kocumarin, a novel
antibiotic isolated from actinobacterium Kocuria
marina CMG S2 associated with the brown seaweed
Pelvetia canaliculata
Farid Menaa
Stem Cells Fluorotronics, Inc USA
Arch Clin Microbiol 2019, Volume:10
DOI: 10.4172/1989-8436-C1-018