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Nanobiotechnology 2018
Page 59
Nano Research & Applications
ISSN: 2471-9838
E u r o S c i C o n C o n f e r e n c e o n
Nanotech & Nanobiotechnology
J u l y 1 2 - 1 3 , 2 0 1 8
P a r i s , F r a n c e
M
agnetic levitation isa technique formeasuring thedensity and themagneticpropertiesof objects suspended inaparamagnetic
feld. Cells can be monitored and studied in an uninterrupted state. Here, we describe a novel magnetic levitation-based
method that can specifcally detect cell membrane-bound and soluble antigens by measurable changes in levitation height that
result from the formation of antibody-coated bead and antigen complex. We demonstrate our method's ability to sensitively
detect an array of membrane-bound and soluble antigens found in blood, including T-cell antigen CD3, eosinophil antigen
Siglec-8, red blood cell antigens CD35 and RhD, red blood cell-bound Epstein-Barr viral particles and soluble IL-6, and validate
the results by flow cytometry and immunofluorescence microscopy performed in parallel. Furthermore, extracellular vesicles
(EVs) can be detected with the addition of anti-CR1 and anti-CD47 in an ELISA based complex. This may provide a quick yes or
no EV/antigen answer. Finally, employing an inexpensive, single lens, manual focus, wif-enabled camera (Melissa), we extend
the portability of our method for its potential use as a point-of-care diagnostic assay with limitless applications. Future studies
are incorporating the use of CCL11 for the prognosis of chronic traumatic encephalopathy. This could lend itself to immediate
diagnosis of conclusions, Alzheimer's disease and dementia.
jtigges@bidmc.harvard.eduDetection and identification of cell bound and soluble
antigens using magnetic levitation: POC detection
John C Tigges, Shulin Lu, Eric Zigon, Vasilis Toxavidis and
Ionita Ghiran
Harvard Medical School, USA
Nano Res Appl 2018, Volume 4
DOI: 10.21767/2471-9838-C2-012