25 / 30
Nanobiotechnology 2018
Page 65
Nano Research & Applications
ISSN: 2471-9838
E u r o S c i C o n C o n f e r e n c e o n
Nanotech & Nanobiotechnology
J u l y 1 2 - 1 3 , 2 0 1 8
P a r i s , F r a n c e
C
ancer chemotherapy remains a challenge due to the mechanisms of resistance of tumour cells and the toxicity of
anticancer drugs. Processes associated with immunogenic cell death may result in the emission of damage-associated
molecular patterns (DAMPs), some are exposed on the plasma membrane, such as heat shock proteins and calreticulin. The
curcumin executes mechanisms that can generate immunogenic cell death in cancer cells, because it can generate an increase
of intracellular calcium that generates a stress in the endoplasmatic reticulum and possibly in the exposition of calreticulin
in the plasma membrane. The photodynamic therapy leads to the generation of reactive oxygen species and thus leading to
immunogenic cell death. In this context, the justifcation for this work is that combined anticancer therapy using curcumin and
aluminium-phthalocyanine chloride nanoemulsion mediated by transcription factor decoy (TFD) can cause intense stress on
cancer cells by promoting immunogenic cell death. The results presented in this study showed that: treatments containing
curcumin and phthalocyanine nanoemulsion were the more toxic to
CT26.WTcells after TFD than free curcumin; in 24 hours
the lipid nanoparticles containing curcumin caused greater increase of granularity in
CT26.WTcells; in 3 hours free curcumin
produces greater accumulation of intracellular calcium than curcumin associated with lipid nanoparticles; in 3 and 24 hours free
curcumin is more internalized than curcumin associated with lipid nanoparticles. Future studies to investigate the generation
other DAMPs (calreticulin, HMGB1, ATP, HSP70 and 90) to prove that curcumin and phthalocyanine are capable of generating an
immune response and are effective against tumours.
rayaneganassin@hotmail.comInduction of immunogenic cell death in tumour
cells sensitized by curcumin and treated with
photodynamic therapy mediated by aluminium-
phthalocyanine chloride nanoemulsion
Rayane Ganassin, Luis Alexandre Muelhmann and Ricardo
Bentes de Azevedo
University of Brasilia, Brazil
Nano Res Appl 2018, Volume 4
DOI: 10.21767/2471-9838-C2-012