18 / 30
Nanobiotechnology 2018
Page 58
Nano Research & Applications
ISSN: 2471-9838
E u r o S c i C o n C o n f e r e n c e o n
Nanotech & Nanobiotechnology
J u l y 1 2 - 1 3 , 2 0 1 8
P a r i s , F r a n c e
T
he expansion of nanoparticles (NPs) and nanomaterials application has drawn increasing concerns about their impact on
environment and human health. Lung is the major target organ during NP exposure. Establishing an
in vitro
lung model
could promote nanotoxicity studies on pulmonary system and paratheatrical development. We proposed a new 3D human lung-
on-a-chip that recapitulates the organ-level structure and functions of
in vivo
lung to investigate nanotoxicity during pulmonary
NP exposure. The microdevice mimicked the alveoli niche including cellular components, 3D extra cellular matrix, and flow
cue to reconstitute the alveolar capillary barrier, allowing tissue/organ level analysis of nanotoxicity during NP exposure (ZnO
and TiO
2
). The results exhibit decreased tight junction protein expression, increased permeability, dose dependent cytotoxicity
under the treatment of NPs, and revealed their varied ability to drive ROS and apoptosis on lung epithelium. This
in vitro
3D ACB
model demonstrated great potential in the study of human pulmonary health, as well as safety assessment for nanoparticles,
environment, food and drugs.
jhqin@dicp.ac.cnA 3D human lung-on-a-chip microdevice for
nanotoxicity testing
Jianhua Qin, Min Zhang and Congxu
Chinese Academy of Sciences, China
Nano Res Appl 2018, Volume 4
DOI: 10.21767/2471-9838-C2-012