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Infectious Diseases 2018
Journal of Prevention and Infection Control
ISSN: 2471-9668
Page 63
June 07-08, 2018
London, UK
8
th
Edition of International Conference on
Infectious Diseases
E
nterobacter cloacae
bacterium is a known symbiont of most
Anopheles
gutmicroflora and nominated as a proper candidate
for paratransgenic control of malaria. Here, we describe a strategy
that uses symbiotic bacteria to deliver anti-malaria effector
molecule to the midgut lumen, thus rendering host mosquitoes
refractory to malaria infection.
Enterobacter cloacae
was
engineered to secret defensin, a natural plant anti-Plasmodium
effector protein. The engineered
E. cloacae
inhibited oocyst
formation of the rodent malaria parasite
Plasmodium berghei
by
up to 92.8% in
Anopheles
stephensi. Significantly, the proportion
of mosquitoes carrying parasites (prevalence) decreased by up to
75%. Interestingly, the wild strain of E.cloacae could inhibit oocyst
formation by up to 72%. These findings provide the foundation for
the use of either wild or geneticallymodified
E.cloacae
bacteria as
a powerful tool to combat malaria.
moshaghi@sina.tums.ac.irFighting malaria using paratransgenic vectors using engineered
Enterobacter cloacae expressing defensin
Mohammad Ali Oshaghi, Hossein Dehghan
and
Seyed Hassan Moosa Kazemi
Tehran University of Medical Sciences, Iran
J Prev Infect Cntrol 2018, Volume 4
DOI: 10.21767/2471-8084-C1-003