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Infectious Diseases 2018 Journal of Prevention and Infection Control
ISSN: 2471-9668 Page 74
June 07-08, 2018
London, UK
8th Edition of International Conference on
Infectious Diseases
Background: In patients with clinical symptoms of respiratory
infection, rapid identification of cases requiring antibiotic
therapy is crucial to avoid development of multiple resistant
bacteria. Neutrophils are important members of innate
immunity and neutrophil extracellular traps (NETs) are
released by neutrophils to control microbial infection. Here we
have developed an affordable, stable, feasible, and accurate
diagnostic tool detecting negatively charged substances (e.g.
oligonucleotides), in sputum from patients with pneumonia,
that showed high and instant reaction to aniline dyes. We
evaluated the ability of this novel test to detect bacterial
infection in cases of pneumonia.
Methods: A colorimetric test was developed. Leftover sputum
samples (n=467) from patients with suspected pneumonia
were blindly tested using the index test. These results were
compared to the ultimate outcomes that were determined
through independent clinical and laboratory assessments
performed by the patient’s physician. The sputum samples
were further analyzed using ELISA, surface plasmon resonance,
SDS-PAGE and ultraviolet-visible spectrophotometry.
Results: The test distinguished pneumonia with high accuracy
(community-acquired and nosocomial pneumonia, n=73 and
controls n=192 without infection, sensitivity 97.2% specificity
78.2%, negative predictive value 96.4% and positive predictive
value 82.6). The results were highly correlated to presence of
hepatocyte growth factor in samples (R=81%) The positive test
result was present even after ultrafiltration of samples in <50
kDa filters. The UV-vis spectrum in samples with positive test
result showed a peak at approximately 265 nm that significantly
differed from the controls.
Conclusions: The presence of proteins and nucleic acids at
the site of bacterial infection might indicate NETs release
from neutrophils. The novel screening test has the potential
to diagnose pneumonia at an early stage and thereby reduce
mortality and morbidity, to guide antibiotic prescription, to
monitor the therapy and to reduce the cost which is especially
vital in poorly equipped centers and rural areas.
A novel instant screening test to distinguish bacterial
respiratory infection
Fariba Nayeri1, Amir Ramezani 2, Lars Eng1 and Annette Theodorsson2
1PEAS Institute, Sweden
2University Hospital in Linköping, Sweden
J Prev Infect Cntrol 2018, Volume 4
DOI: 10.21767/2471-8084-C1-003