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Immunology 2018
J u l y 0 5 - 0 7 , 2 0 1 8
V i e n n a , A u s t r i a
Page 40
Journal of Clinical Immunology and Allergy
ISSN 2471-304X
1 5
t h
I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology
Objective:
To investigate the
in vitro
effects of ellagic acid on vaginal innate
immunity.
Methods:
Vaginal epithelial cell culture was performed in the presence or
absence of ellagic acid. Expression of human hBD2 and SLPI was determined at
both transcriptional and translational levels. In addition, expression of various
cytokines and chemokines including IL-2, IL-4, IL-6, IL-8, IL-10, CCL-2, CCL-5,
TNF- α, IL-1β, and IFN-γ were investigated using Luminex assay. Cytotoxicity of
ellagic was also determined using MTT assay.
Results:
The expression of hBD2 mRNA was significantly increased at both
transcriptional and translational levels in response to ellagic acid (p<0.05).
SLPI mRNA expression was significantly increased in the presence of ellagic
acid. The expression of IL-2 was induced in response to ellagic acid in a
dose-dependent manner. In contrast, no changes in the expression of other
cytokines/chemokines were observed. No cytotoxicity of ellagic acid was
noted on vaginal epithelial cells.
Conclusions:
We conclude that vaginal epithelial cells can recognize a plant-
derived compound. Innate immune factors produced by vaginal epithelial
cells are differentially expressed in response to ellagic acid. Thus, plant-
derived compounds such as ellagic acid may be useful to be developed as an
immunomodulatory agent to improve vaginal health.
Biography
Wipawee Nittayananta has obtained her Doctoral degree from
Freie Universitaet Berlin, Germany and completed her PhD from
the University of Washington, USA. She has published more
than 40 papers in reputed journals and has been serving as an
Editorial Board Member of repute
.
wipawee.ni@psu.ac.thEllagic acid modulates the expression of vaginal innate immune
mediators
Aornrutai Promsong
1
, Surada Satthakarn
2
and Wipawee Nittayananta
3
1
Princess of Naradhiwas University, Thailand
2
University of Phayao, Thailand
3
Thammasat University, Thailand
Wipawee Nittayananta et al., Insights Allergy Asthma Bronchitis 2018, Volume: 4
DOI: 10.21767/2471-304X-C1-002