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Immunology 2018
J u l y 0 5 - 0 7 , 2 0 1 8
V i e n n a , A u s t r i a
Page 38
Journal of Clinical Immunology and Allergy
ISSN 2471-304X
1 5
t h
I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology
I
n the context of vaccine immunogenicity, it is important to evaluate whether
co-culturing candidate adjuvants can promote antigen uptake by antigen-
presenting cells and lead to enhance vaccination feasibility. Here, we
explored mechanistic reasoning toward embracing the interface between the
physicochemical and biological signatures for core oil selection in vaccine
immunogenicity. Our results showed that treatment of dendritic cells (DCs)
and splenocytes with a squalene-based emulsion (referred as SqE) induced
reactive oxidative species (ROS) production and resulted in an increase
in apoptotic and necrotic cells in a concentration- and time-dependent
manner. Furthermore, DCs co-cultured with cellular debris of SqE-pretreated
splenocytes resulted in a higher level of ovalbumin (OVA) antigen uptake by
DCs than those co-cultured with untreated splenocytes. Interestingly, the
potency was rather attenuated when splenocytes pretreated with a typical ROS
inhibitor, N-acetyl-cysteine. Notably, SqE possesses a high impact on eliciting
ROS-mediated antigen uptake compared with a squalane-based emulsion
(SqA). Concordantly, immunogenicity studies have shown that SqE is better
able than SqA to activate antigen-presenting cells, and to enhance antigen-
specific T-cell immunity. Accordingly, our results highlight the importance of
unsaturated squalene core oil in the adjuvant activity of emulsions and offer
insight into the design and development of vaccine adjuvants.
Biography
Ming-Hsi Huang obtained his PhD degree in 2004 in Materials
Chemistry fromUniversity Montpellier I in France. He worked as
a Postdoctoral fellow and an Assistant Investigator in Vaccine
Research and Development Centre of NHRI. Currently, he is
an Associate Investigator in NIIDV of NHRI, Taiwan. He has
publishedmore than 30 SCI journal articles, 1 book chapter, and
granted 5 patents
.
huangminghsi@nhri.org.twUnsaturated squalene content in emulsion vaccine adjuvants plays a
crucial role in ROS-mediated antigen uptake and cellular immunity
Chung-Hsiung Huang, Chiung-Yi Huang and Ming-Hsi Huang
National Institute of Infectious Diseases and Vaccinology, Taiwan
Ming-Hsi Huang et al., Insights Allergy Asthma Bronchitis 2018, Volume: 4
DOI: 10.21767/2471-304X-C1-002