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E u r o S c i C o n C o n f e r e n c e o n
Chemistry
2018
Chemistry 2018
Journal of Organic & Inorganic Chemistry
ISSN 2472-1123
F e b r u a r y 1 9 - 2 0 , 2 0 1 8
P a r i s , F r a n c e
Page 51
Cataract development among diabetic patients is a real problem nowadays.
This opacification of the lens appears due to the high glycaemic levels,
when glucose cannot be metabolized through the normal pathway due to its'
saturation, so it is metabolized through an additional one called the polyol
pathway, where under the action of aldose reductase it is transformed into
sorbitol, and further the sorbitol under the action of sorbitol dehydrogenase
is transformed to fructose. Both sorbitol and fructose are inactive compounds
and possess osmotic properties. Synthetic compounds, although with a
high capacity of inhibition have proved to have serious side effects. Natural
compounds could be an alternative for aldose reductase inhibition. The
present study was undertaken to evaluate the prevention of cataractogenesis
in vitro by Vaccinium extracts obtained from the fruits and leaves collected
from Vaccinium myrtillus and Vaccinium corymbosum. Total polyphenols,
total monomeric anthocyanins and tannins content were determined
spectrophotometrically. Chlorogenic acid was quantitatively determined by
HPLC-DAD. The study was performed on chicken lenses incubated for 72
hours in Roti@CELL-Medium (with glutamine and HEPES), 55 mM glucose and
Vaccinium extracts. The lens opacity was graded on a 4-stage scale. None of
the lenses incubated with Vaccinium myrtillus leaves extract had shown signs
of opacity. Vaccinium corymbosum leaves extract showed a lower protection
against opacification. Fruit extracts did not prevent the opacification of the
lenses. The protective effect of leaves extracts on lens is correlated with
the chlorogenic acid content, which is considered to be an aldose-reductase
inhibitor.
Biography
Ruxandra Ștefănescu (Braic) is a PhD student from the Univer-
sity of Medicine and Pharmacy Tîrgu Mureș. Her main interests
are Phytotherapy, Phytopharmacology, Pharmacology, Pharma-
cognosy and Analytical Chemistry.
Polyphenolic compounds from Vaccinium species act as aldose-
reductase inhibitors?
Ruxandra Ștefănescu (Braic), Silvia Imre, Camil Vari, Eszter
Laczkó-Zöld, Alexandra Groșan and Maria Dogaru
University of Medicine and Pharmacy of Tîrgu Mureș, Romania
Ruxandra Stefănescu (Braic), J Org Inorg Chem 2018, Volume: 4
DOI: 10.21767/2472-1123-C1-003