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Page 49
Volume 4
December 10-12, 2018
Rome, Italy
Nano Research & Applications
ISSN: 2471-9838
Advanced Materials 2018
Nano Engineering 2018
JOINT EVENT
22nd International Conference on
Advanced Materials
and Simulation
&
22 nd Edition of International Conference on
Nano Engineering &
Technology
Nanotechnology-based cargo delivery to cells is a promising
assay due to its efficiency and biocompatibility. Time-
consuming and uncontrollable deliver amount are still
thresholds in related research. In this study, we design a
nanopillar-based platform for liposome delivery in vitro.
Thermoreponsive copolymer (p(NIPAm-co-AEMA)) is grafted
from silicon nanowire (SINW) as a polymer nano brush to
equip temperature-controllable liposome conjugations of the
SINW. A liposome is introduced onto the polymer nano brush
to form a nano-by-nano interface and is released through
thermal-stimuli to generate a high local concentration for
cellular uptake. Cryo-TEM images show that liposomes can
attach to the polymer nano brush to form liposome-tethered
nanopillars. Fluorescence quantifications suggest that up to
90% of the attached liposomes can be released with intactness.
Furthermore, HEK 293T cell and calcein-loaded liposome
are employed to investigate the cellular uptake kinetics.
We found that the cellular uptake kinetic of HEK 293T cell is
highly correlated to temperature stimulus in the nano-by-nano
system. Fluorescence intensity difference quantified by flow
cytometry indicate elevated cellular uptake efficiency, which is
more than 10-fold increments at 4-hour incubation. Confocal
images show that the liposomes stay at cytoplasm instead of
lipid membrane after cellular uptake. In conclusion, we could
deliver liposome to cells efficiently with the designed system
and we hope these efforts could open a new era in biological
engineering and tissue engineering.
Biography
Mr. Gautam received his master degree in Chemical Science from Institute
of chemical technology (ICT), Mumbai, India. After completing his master
degree he worked 2 years as Research Assistance at Aditya Birla Science
and Technology Company. He is currently a pursuing his Ph.D. study at Insti-
tute of Chemistry, Academia Sinica, Taiwan.
Developing “SMART” Nano by Nano System for
Efficient Liposome Delivery in vitro
Bhaskarchand Gautam1,2,3, Chun-Hao Luo 1, Hua-De Gao1 , Hsian-Rong Tseng,#
Hsien-Ming Lee1,2* and Hsiao-hua Yu1,2*
1Institute of Chemistry, Academia Sinica, Nankang, Taipei 115, Taiwan; 2Taiwan International
Graduate Program (TIGP), Sustainable Chemical Science and Technology (SCST), Academia
Sinica, Taipei, Taiwan, #Department of Molecular and Medical Pharmacology, California Nano
System Institute University of California, Los Angeles 570 Westwood Plaza, Los Angeles,
CA90095-1770,USA
Bhaskarchand Gautam et al., Nano Res Appl 2018, Volume 4
DOI: 10.21767/2471-9838-C7-027