25 / 31
Page 46
Volume 4
December 10-12, 2018
Rome, Italy
Nano Research & Applications
ISSN: 2471-9838
Advanced Materials 2018
Nano Engineering 2018
JOINT EVENT
22
nd
International Conference on
Advanced Materials
and Simulation
&
22
nd
Edition of International Conference on
Nano Engineering &
Technology
E
pithelial-to-mesenchymal transition (EMT) is a highly
orchestrated process motivated by the nature of physical
and chemical compositions of the tumor microenvironment
(TME). The role of the physical framework of the TME in
guiding cells toward EMT is poorly understood. To investigate
this, breast cancer MDA-MB-231 and MCF-7 cells were
cultured on nanochips comprising tantalum oxide nanodots
ranging in diameter from 10 to 200 nm, fabricated through
electrochemical approach and collectively referred to as
artificial microenvironments. The 100 and 200 nm nanochips
induced the cells to adopt an elongated or spindle-shaped
morphology. The key EMT genes, E-cadherin, N-cadherin,
and vimentin, displayed the spatial control exhibited by the
artificial microenvironments. The E-cadherin gene expression
was attenuated, whereas those of N- cadherin and vimentin
were amplified by 100 and 200 nm nanochips, indicating the
induction of EMT. Transcription factors, snail and twist, were
identified for modulating the EMT genes in the cells on these
artificial microenvironments. Localization of EMT proteins
observed through immunostaining indicated the loss of cell−
cell junctions on 100 and 200 nm nanochips, confirming the
EMT induction. Thus, by utilizing an in vitro approach, we
demonstrate how the physical framework of the TME may
possibly trigger or assist in inducing EMT in vivo. Applications
in the fields of drug discovery, biomedical engineering, and
cancer research are expected.
Recent Publications
1. Dhawan U, Sue MW, Lan KC, Waradee B, Huang PH,
Chen YC, Chen PC, Chen WL (2018) “Nanochip
induced Epithelial to mesenchymal transition: Impact
of Physical microenvironment on cancer metastasis”.
ACS Applied Materials and Interfaces.
2. Dhawan U, Wang SM, Chu YH, Huang GS, Lin YR, Hung
YC, Chen WL. “Nanochips of Tantalum oxide nanodots
as artificial microenvironments for monitoring ovarian
cancer progressiveness” Scientific Reports.
3. Dhawan U, Lee CH, Huang CC, Chu YH, Huang GS, Lin
YR, Chen WL. (2015) “Topological control of Nitric
oxide secretion by Tantalum oxide nanodots arrays”.
Journal of Nanobiotechnology.
Biography
Dr. Udesh earned his Ph.D. in Biomedical Engineering from the Department
of Materials Science and Engineering at National Chiao Tung University,
Taiwan. He is currently a postdoctoral fellow at the Institute of Chemistry,
Academia Sinica, Taiwan. He has published several articles in the field of
Biomaterials and cancer biology. He serves as an editorial board member
for the journal SF journal of Materials and Chemical Engineering and is the
managing editor for the journal Frontiers in Bioscience. His research inter-
ests include engineering nanostructured biomaterials for cancer therapy,
drug discovery and drug development.
udeshdhawan.91@gmail.comNanochip-induced epithelial to mesenchymal
transition: impact of physical microenvironment on
cancer metastasis
Udesh Dhawan
1
, Ming-Wen Sue
2
, Kuan Chun Lan
2
, Waradee Buddhakosai
2
, Pao
Hui Huang
2
, Yi Cheng Chen
3
, Po Chun Chen
3
, Wen Liang Chen
2
1
Department of Materials Science and Engineering, National Chiao Tung University, 1001 Uni-
versity Road, Hsinchu, Taiwan, 300, ROC
2
Department of Biological Science and Technology, National Chiao Tung University, 1001 Uni-
versity Road, Hsinchu, Taiwan, 300, ROC
3
Department of Materials and Mineral Resources Engineering, National Taipei University of
Technology, 1, Section 3, Zhongxiao E. Rd, Taipei, Taiwan, 10608, ROC.
Udesh Dhawan et al., Nano Res Appl 2018, Volume 4
DOI: 10.21767/2471-9838-C7-027