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A p r i l 2 2 - 2 3 , 2 0 1 9
A t h e n s , G r e e c e
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Archives of Clinical Microbiology
ISSN: 1989-8436
Virology and Infectious Diseases 2019
EuroSciCon Conference on
Virology and Infectious Diseases
I
nvasive infections caused by methicllin resistant
Staphylococcus aureus
and coagulaso-negative Staphylococci (MRSA/MRSCoN) require fast
laboratory detection and start of adequate treatment. The aim of this
study was to develop a new faster protocol for direct detection of MRSA/
MRSCoN in blood cultures and in abscess punctures. For this purpose
were used polymerase-chain reaction (PCR) by primers for species
specific identification of S aureus and methicllin resistance gene
mecA
.
We examined 85 growth-positive BACTEC blood cultures and 56 abscess
punctures by routine microbiological assay and simultaneous PCR
detection of MRSA/MRSCoN. The speci city of the PCR was evaluated by
using DNA from another 16 microbial species for negative controls. We
determined the susceptibility to methicillin by disc cefoxitine according
EUCAST 2019 criteria and minimum inhibitory concentration (MIC)
of oxacillin against the S aureus isolates using the E-test. In the blood
cultures, the two methods detected near 40% MRSA, resp. 94% MRCoNS.
In the punctures, the PCR assay identified near 20% MRSA. The PCR
and the routine microbiological results for the blood samples are fully
consistent but the new method was faster (only a few hours were need).
Among the punctures, there were five PCR MRSA positive and culture
negative samples. The new PCR protocol was more sensitive and again
faster for detecting MRSA from abscess punctures than the routine
microbiological techniques. This molecular-genetic test will speed up the
right choice of empirical therapy, which is extremely important for saving
patients’ lives
Biography
Virna Maria Tsitou has completed her Specialty Doctor
of Medicines from Medical University of Sofia- Bulgaria in
2011. She worked in Emergency Department in “Prevent
DCC” hospital from 2009-2012. She has also worked as
Intern in Dermatology Department in “Tokuda Hospital” in
Sofia Bulgaria from 2010-2013 and Intern in Microbiology
Department in “ISUL-TSARITSA IOANNA” hospital in Sofia
Bulgaria from 2013-2014. She has served as a Specialist
Microbiologist in the Department of Medical Microbiology
in Medical University Sofia–Bulgaria from 2014-2018. She
is working as an Assistant Professor in the Department of
Medical Microbiology in Medical University Sofia–Bulgaria
since 2016. She is fluent in Greek, English and Bulgarian
language. Her participation in Congresses is very active
because she wants to share her research findings with other
scientists from the Microbiological Community and also keep
up with the scientific innovations in Bulgaria but also abroad.
Maria-Tsitou@hotmail.co.ukPCR detection of
Staphylococcus aureus
and
mecA
gene in
patients with invasive infections
Virna Maria Tsitou, Ivan Mitov and Raina Gergova
Medical University Sofia, Bulgaria
Virna Maria Tsitou et al., Arch Clin Microbiol 2019, Volume:10
DOI: 10.4172/1989-8436-C1-018