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Archives of Clinical Microbiology
ISSN: 1989-8436
Virology and Infectious Diseases 2019
EuroSciCon Conference on
Virology and Infectious Diseases
Background & Aim:
Bovine tuberculosis is one of the most important
zoonotic diseases in Bovidae. Humans and animals that transit to the farm
can transfer
Mycobacterium
to the cattle. Hence, the aim of this study is
to evaluate the possible role of cats in transferring the
Mycobacterium
infection in dairy farms.
Methods:
From a dairy cattle farm with more than 20% infection of
Mycobacterium
, seven cats were captured and their gastric juice cultured
in the LJ and LG medium. The acid-fast staining of the isolates prepared to
identify
Mycobacterium
and PCRs were carried out afterwards.
Results:
Five out of seven cultureswere positive in direct smear by acid fast
staining and in PCR-16SrRNA, which indicates that the above-mentioned
isolates belong to the
Mycobacterium
genus. Also, positive PCR-IS6110
confirmed that the isolate species are identified as
Mycobacterium
tuberculosis complex. Currently, we are conducting sequencing for the
exact identification of these isolates.
Conclusion:
Animals such as mice and cats that live in the farm can
harbour
Mycobacterium
. In this study, it has been proven that cats certainly
transfer
Mycobacterium
to the cattle farms.
Biography
Khashaiar Mansouri is currently studying Doctor of veterinary
medicine at the Islamic azad university Garmsar branch.
His Research focus is on zoonotic diseases particularly
Mycobacterium
in cat and mice, as well as Bukholderia mallei
in Guinea pig. Recently, he has given poster presentation in
the 19th international and Iranian congress of microbiology.
khashaiar.mansouri.dvm@gmail.comIsolation and identification of
Mycobacterium
from captured
cats belonging to tuberculosis infected farms
Khashaiar Mansouri
1
and Nader Mosavari
2
1
Islamic Azad University-Garmsar Branch, Iran
2
Razi Vaccine and Serum Research Institute-AREEO, Iran
Khashaiar Mansouri et al., Arch Clin Microbiol 2019, Volume:10
DOI: 10.4172/1989-8436-C1-018