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Archives of Clinical Microbiology
ISSN: 1989-8436
Virology and Infectious Diseases 2019
EuroSciCon Conference on
Virology and Infectious Diseases
C
oronaviruses have been described in many mammalian species,
including humans, and have gained prominence in zoonotic preserve
viruses, especially after SARS-coronavirus (Severe Acute Respiratory
Syndrome) and MERS-coronavirus (Middle East Respiratory Syndrome)
outbreaks. Bovine infectious coronaviruses (BCoV), often have been
described as an important cause of diarrhea, especially in newborn
calves, in many countries including Turkey. Besides the newborn
calves’ diarrhea, BCoVs also causes digestive system infections (winter
dysentery) and respiratory system infections in adult cattle. In this study,
we aimed to investigate the presence of coronavirus as an aetiological
agent in cattle with respiratory disorders or diarrhea and to analyze the
relationship with other coronaviruses reported before. Therefore, 50
feces samples and 50 nasal swab samples from calves with diarrhea and
adult cattle with respiratory disorders, respectively, were tested for the
presence of coronavirus. Among the nasal samples and fecal samples,
5 (10%) and 8 (16%) were positive based on the results for BCoV N gene
by nested RT-PCR (Reverse Transcription-Polymerase Chain Reaction).
Sequence analysis was performed via service procurement. Comparison
analysis completed with the web tool NCBI-BLAST, BioEdit and MEGA-X
programmes for phylogenetic analysis. Our results demonstrated that
BCoV is a pathogen having a role in mentioned disorders alone or
along with other possible viral or bacterial pathogens. The phylogenetic
analysis of N gene region of detected BCoVs indicated that all of them
were related to the reference strain Mebus. Further studies on the
epidemiology and molecular characterization of different gene regions
will provide a better understanding of the importance of BCoVs in these
cases and the pathogenetic mechanism that provides sensitivity to
different systems. Thus, the analysis of S gene, coding the protein which
has several important functions during virus-host interaction are still
continuing for BCoVs detected in this study.
Biography
Secil Sevinc is a PhD student and also Research Asistant
at Faculty of Veterinary Medicine, Department of Virology,
Ankara University. She is a Member of the Society of
Veterinarians inTurkey. Also she is aMember of the Society of
Veterinary Microbiology in Turkey.
sevinc@ankara.edu.trThe detection of bovine coronaviruses in fecal and nasal
specimens from cattle in Turkey
Secil Sevinc and Feray Alkan
Ankara University, Turkey
Secil Sevinc et al., Arch Clin Microbiol 2019, Volume:10
DOI: 10.4172/1989-8436-C1-018