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Plant Genomics 2019
June 13-14, 2019
Berlin, Germany
Asian Journal of Plant Science & Research
ISSN: 2249-7412
Page 39
Plant Genomics
5
th
Edition of International Conference on
AJPSKY 2019, Volume 09
De Novo sequencing and hybrid assembly of biofuel
crop
Jatropha curcas
Ls Insights on gene annotation,
comparative genomics and transcriptome analysis with
identification of quantitative trait loci for geminivirus
resistance
Nagesh Kancharla
Reliance Industries Limited, India
J
atropha curcas
is an important perennial, drought
tolerant plant identified as a potential biodiesel crop.
We report here the hybrid de novo genome assembly
of
Jatropha curcas
generated using Illumina and
PacBio sequencing technologies and identification of
quantitative loci responsible for geminivirus resistance. In
this study, we generated scaffolds of 265.7 Mbp in length
which is corresponding to 84.8% of the gene space using
BUSCO analysis. Additionally, 96.4% of predicted protein-
coding genes were captured in corresponding tissues
transcriptome data, which reconfirms the accuracy of
the assembled genome. The genome was utilized to
identify 12,103 dinucleotide repeat SSR markers, which
were exploited in genetic diversity analysis to identify
genetically distinct lines. A total of 250, polymorphic SSR
markers were employed to construct a genetic linkage
map for Jatropha Mosaic Virus (JMV) resistance using
interspecific F2 mapping population involving
Jatropha
curcas
and
Jatropha integerrima
as parents. QTL analysis
showed the identification of three minor Quantitative
Trait Locus (QTL) for JMV resistance and validated in
an alternate recipient genetic background F2 mapping
population. These validated QTLs were utilized in Marker
Assisted Breeding (MAS) for developing
JatrophaMosaic
Virus resistance hybrids in
Jatropha
. Comparative
genomics of oil-producing genes across selected oil
producing species revealed 27 conserved genes and
2,986 orthologous protein clusters in
Jatropha
. This
reference genome assembly gives an insight into the
understanding of the complex genetic structure of
Jatropha
and serves as source for the development of
agronomically improved virus resistant and oil producing
species.