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Pharmacognosy 2019
March 11-12, 2019
London, UK
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 42
Pharmacognosy and
Medicinal Plants
7
th
Edition of International Conference on
Soleya Dagnon et al., Am J Ethnomed 2019, Volume 6
DOI: 10.21767/2348-9502-C1-009
Mass spectrometric elucidation of polyphenol constituents
in
Vernonia amygdalina
Del.
Soleya Dagnon
1
, Dimitar Bojilov
1
, Zlatomira Novkova
1
, Paraskev Nedialkov
2
and
Kristian Kouassi
3
1
University Paisii Hilendarski, Bulgaria
2
Medical University of Sofia, Bulgaria
3
Nangui Abrogoua University, Cote d’Ivoire
V
ernonia amygdalina
Del. (VA), family Asteraceae
is a well-known shrub in tropical countries
because of its nutritive and medicinal properties. The
antioxidant and anticancer therapeutic effects of VA
were related to polyphenol content. For this purpose,
mass spectrometric investigation was conducted
aiming at characterizing the polyphenol composition
of Vernonia amygdalina. Previous publications on the
polyphenol constituents in VA raise some questions
on the component composition caused by inadequacy
of the data. It seems necessary to prove the presence
of luteolin 7-O-glycosides and the aglycone luteolin.
Monocaffeoyl-, dicaffeoyl- and tricaffeoylquinic
acids, as well as, feruloyldicaffeoylquinic acid were
also reported. In this study the polyphenols were
extracted using methanol in an ultrasound bath for
40 min at room temperature (25°C). The extract was
evaporated to dryness, resolved in water and divided
into chloroform fraction and ethyl acetate fraction. The
compounds in both the fractions were characterized
by HPLC-PDA. Structural elucidation was performed
on Orbitrap UHPLC mass spectrometer. Two flavone
aglycones luteolin and apigenin were identified in the
chloroform fraction. In the ethyl acetate fraction six
flavone glycosides luteolin 7-O-glucoside, luteolin
7-O-glucuronide, luteolin 4’-O-glucoside, apigenin
7-O-rutinoside, apigenin 7-O-glucoside and apigenin
7-O-glucuronide were found, as well as mono and
dicaffeoylquinic acids and feruloylquinic acid-O-
glycoside. The caffeoylquinic acids (chlorogenic 1,5-3,5
and 4,5-dicaffeoylquinic acids), luteolin 7-O-glucoside,
luteolin 7-O-glucuronide and luteolin are the main
compounds composing the polyphenol complex of VA.
The largest percentage of them was due to 1,5 and 3,5
dicaffeoylquinic acids. Furthermore, the presence of
luteolin 4’-O-glucoside, apigenin 7-O-rutinoside and
feruloylquinic acid-O-glycoside as minor constituents
in
Vernonia amygdalina
is reported for the first time.