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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 86
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
T
he purpose of this research was to accomplish chemical
study, antioxidant analysis and evaluation of the larvicidal
potential against Aedes aegypti larvae of essential oil from the
leaves of
O. basilicum
Linn. The research was carried out in the
Pharmacognosy and Phytochemistry Laboratory, Department
of Biologicaland Health Sciences, Federal University of Amapá
(UNIFAP), between July 2013 and March 2014. Arthropoda
Laboratory, Department of Biological andHealthSciences, Federal
University of Amapá (UNIFAP) between September 2013 and
March 2014. The essential oil was obtained by hydrodistillation;
the identification and quantification of components was achieved
with the use of GC-MS analysis. The antioxidante activity was
evaluated by the method of sequestration of DPPH. The essential
oil was tested in the third larval state of the development of the
mosquito Aedes aegypti. The third larval instar were exposed to
different concentrations of the oil (500, 400, 300, 200 and 130
ppm) in triplicates. Chromatographic analysis identified that the
major constituents found in essential oil of
O. basilicum
were
limonene (13%), 1,8-cineole (15%), linalool (20%) and methyl
chavicol (45%). In trialsof free radicalssequestration, theessential
oil showed (AA%) 67.35±1.11 in the highest concentration
and inhibitory concentration, IC50 value of 61.517 mg/mL. The
essential oil of
O. basilicum
showed larvicidal potential with CL50
of 67.22 ppm. A more detailed study should be done to verify the
larvicidal potential and biological mechanismof action, as several
authors claimed that the constituent of essential oils affect the
nervous system of the mosquito Aedes aegypti and the action
mechanism is not yet fully elucidated. New studies demand the
development of tests using samples of lower concentrations to
verify the degree of toxicity in other animal species, including
man, and preparation of formulations that may function as a
natural alternative to combat mosquito larvae.
Recent Publications
1. Almeida S S M S (2017) Chemical composition and
in
vitro
antioxidant, cytotoxic, antimicrobial, and larvicidal
activities of the essential oil of Mentha piperita L.
(Lamiaceae). The Scientific World Journal 2017:1–8.
2. Almeida S S M S (2017) Mineral composition of leaves,
ethanolic leaf extract and infusions of A. occidentale
L. from Amazon in Northern Brazil. Mintage Journal of
Pharmaceutical E Pharmaceutical Research 6:8–11.
3. Almeida S S M S (2016) Chemical composition: an
antioxidant, cytotoxic and microbiological activity of the
essential oil from the leaves of Aeollanthus suaveolens
Mart. ex Spreng. Plos One 1:1–10.
4. Almeida S S M S (2016) Antioxidant effect of plant
extracts of the leaves of Tithonia diversifolia (Hemsl.) A.
Gray on the free radical DPPH. Journal of Chemical and
Pharmaceutical Research 8:1182–1189.
5. Almeida S S M S (2016) Larvicide and antioxidant
activity of the ethanol crude extract from the stem bark
of Pseudoxandra cuspidata (Annoaceae). Journal of
Chemical and Pharmaceutical Research 8:841–846.
Chemical study, antioxidant analysis and evaluation of the
larvicidal potential against Aedes aegypti larvae of essential
oil of Ocimum basilicum Linn.
Sheylla S M S Almeida, Rosany L Martins, Ana L F Farias, Alex B L Rodrigues
and
Érica M Rabelo
Universidade Federal do Amapá, Brazil
Sheylla S M S Almeida et al., Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-006