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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 70
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
Antiproliferative activity of three sesquiterpenes on human
bladder cancer cell lines
Mila Radan, Franko Burcul
and
Vedrana Cikes-Culic
University of Split, Croatia
S
esquiterpenes are C15-terpenoids built from three isoprene
units. These compounds are commonly found in higher
plants, marine organisms and fungi. Naturally, they occur
as hydrocarbons or in oxygenated forms including lactones,
alcohols, acids, aldehydes, and ketones. Over the last decade,
sesquiterpenes have attracted significant attention because
of the roles they play in biological systems and their diverse
biological activities, such as anti- inflammatory, antibacterial,
antioxidant and anti-carcinogenic. The antiproliferative activity
of three sesquiterpenes (thuyopsene, nerolidol and farnesol)
was evaluated on three lines of human bladder cancer (T24,
UM-UC-3, TCCSUP,) using MTT assay. Thuyopsene is one of the
major sesquiterpene constituents of cedarwood, while farnesol
and nerolidol can be found in the essential oils of many types
of plants and flowers. The tested compounds showed different
antiproliferative activity on the cancer cell lines. Farnesol showed
the highest impact on T24 cells growth (52% growth inhibition) at
concentration of
ϱϲ
μM, while thuyopsene showed the strongest
growth inhiďition of
ϱϬ
% and 27% on UM-UC-3 and TCCSUP cell
line at concentration of
ϭϳϭ
and
ϭϮϮ
μM, respectively. Generally,
nerolidol showed the lowest antiproliferative potential on the
cancer cells. In order to determine type of cell death induced by
treatment, Annexin-V-FITC assay was used for the detection of
apoptosis by flow cytometry. The results obtained for these three
sesquiterpenesopenaperspective for their useasantiproliferative
agents.
mradan@ktf-split.hrMila Radan et al., Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-006