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Volume 3, Issue 3 (Suppl)
J Obes Eat Disord
ISSN: 2471-8203
Obesity Medicine 2017
October 30-31, 2017
October 30-31, 2017 Bangkok, Thailand
15
th
International Conference on
Obesity Medicine
Protective role of soybean extract from H
2
O
2
-oxidative stress and cell death in human keratonicyte
HaCaT Cells
Yoon-Mi Lee
1
, Hyun-Min Park
1
, Sooji Song
1
, Kyung-Jin Yeum
1
and Yu Young Lee
2
1
Konkuk University, Republic of Korea
2
National Institute of Crop Science, Republic of Korea
O
xidative stress is closely related to incidence of various skin diseases. The soybean has been known as beneficial food that
is demonstrated to possess a variety of antioxidants. Here, we examined whether soybean extract had a protective role
against oxidative stress-induced cell death in human keratinocyte HaCaT cells. Firstly, we determined bioactive components
in the soybean extract (Cheongja#3 black soybeans) using ultra performance liquid chromatography (UPLC) analysis. We
found that 1.38 μg of γ-tocopherol and 9.738 ng of lutein existed in 100 mg of soybean extract. Soybean-treatment markedly
decreased hydrogen peroxide-generated intracellular reactive oxygen species (ROS) levels at 100 μg/ml without no cytotoxicity.
Furthermore, soybean extract protected cells fromH
2
O
2
-induced cell death by assessment of MTT assay and caspase activities.
From these results, antioxidant function of soybean extract could be potential for the protection of human keratinocytes
HaCaT cells from oxidative stress and triggered cell death.
Biography
Yoon-Mi Lee, Ph.D is a post doctor at the department of food bioscience, Konkuk University, South Korea. She received her BS degree in the Department of
Bioscience, Sungkunkwan University and Ph.D degree in the College of Medicine, Seoul National University, South Korea. While pursuing her Ph.D. degree, she
was studying identifications of drugs targeting cancer and characterizing their molecular mechanisms in cells and animal models. She is currently focusing on
identifying efficacy of food bioactives in the prevention and treatment of various chronic diseases including metabolic disorders.
yoonmilee@kku.ac.krYoon-Mi Lee et al., J Obes Eat Disord 2017, 3:3
DOI: 10.21767/2471-8203-C1-006