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Medchem & Toxicology 2018
Page 51
Journal of Organic & Inorganic Chemistry
ISSN: 2472-1123
A n n u a l C o n g r e s s o n
Medicinal Chemistry,
Pharmacology and toxicology
J u l y 3 0 - 3 1 , 2 0 1 8
Am s t e r d a m , N e t h e r l a n d s
The research included the isolation of myeloperoxidase and glutamate
dehydrogenase from tumour lung tissue using different biological
techniques. These included: ammonium sulphate precipitation, dialysis, gel
filtration chromatography on and Sephadex G–200. The results predicted that
specific activity and the number of fold of purification were (42.59 U/ml) and
(36.3) respectively for partially purified myeloperoxidase, While it was (22.69
U/ml) and (44.1) respectively for partially purified glutamate dehydrogenase.
Furthermore, the comparative molecular weight of the partially isolated
myeloperoxidase and glutamate dehydrogenase was (150.3 kDa) and (332.3
kDa) respectively using gel filtration chromatography. The study showed that
the optimum conditions of myeloperoxidase were obtained at the first minute
using sodium citrate (0.1 M) as buffer at pH (5.5), at a temperature (45 ºC)
and (14 mM) of ο-Dianisidin as substrate. It was found that Vmax and Km have
the values of (18.86 U/ml) and (2.69 mM) respectively. Finally, the optimum
conditions of glutamate dehydrogenase were obtained using Tris-HCl (100
mM) as a buffer at pH (8.6), (40 C°) and (35 mM) of glutamate as a substrate.
It was found that Vmax and Km have the values of (14.1 U/ml), (16.56 mM)
respectively.
Biography
Mahmoud A. M. Fakhri has completed his PhD in Biochemistry
(Clinical Chemistry) from Mosul University. He is a Lecturer in
Biochemistry field (Biomolecules, Metabolism, Biotechniques,
and Biostatistics) at Biophysics Department/ Science College/
Mosul University. He is the Supervisor of two Postgraduate
students (Biochemistry and Clinical Chemistry field) in Science
College / Mosul University. He has published more than 7
papers in reputed journals and has been serving as a Member
in Iraqi Society of Nanotechnology.
Isolation and study of myeloperoxidase and glutamate
dehydrogenase enzymes from tumour pulmonary tissue
Mahmoud A M Fakhri and Raged A Hamo
University of Mosul, Iraq
Mahmoud A M Fakhri et al., J Org Inorg Chem 2018, Volume 4
DOI: 10.21767/2472-1123-C3-009