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Medchem & Toxicology 2018
Page 35
Journal of Organic & Inorganic Chemistry
ISSN: 2472-1123
A n n u a l C o n g r e s s o n
Medicinal Chemistry,
Pharmacology and toxicology
J u l y 3 0 - 3 1 , 2 0 1 8
Am s t e r d a m , N e t h e r l a n d s
T
embotrione, a triketone herbicide used for post-emergence weed
control in corn, as a potential endocrine disrupting chemical
can disrupt reproductive and sexual development by affecting
sex hormones. This could be especially detrimental during early
development with adverse effects until adulthood. We evaluated the
effects of tembotrione exposure during gestation and/or lactation
on estradiol and testosterone levels in female and male offspring 24
hours after birth (newborns), at weaning (21-day-old pups) and at the
beginning of puberty. Wistar rats were exposed orally to tembotrione
doses relevant to real human exposure: 0.0004, 0.0007 and 4.0 mg/kg
bw/day and ethinylestradiol as positive control (PC) during the entire
gestation and/or lactation period. Hormone levels were measured in
offspring serum by enzyme-linked immunosorbent assay. Oestradiol
decreased at 4.0 mg/kg bw/day compared to negative control (NC)
and PC, 0.0004 and 0.0007 mg/kg bw/day in newborns and increased
at 4.0 mg/kg bw/day compared to 0.0004, 0.0007 mg/kg bw/day and
PC in 21-day-old pups exposed only during lactation. Exposure during
gestation and lactation increased oestradiol at 0.0007 mg/kg bw/
day compared to PC and 0.0004 mg/kg bw/day in 21-day-old pups. In
pubertal female offspring, oestradiol decreased at 0.0004 mg/kg bw/
day compared to PC. Testosterone increased in newborns at 0.0004
mg/kg bw/day compared to NC, PC and 0.0007 mg/kg bw/day and in
21-day-old pups exposed only during lactation compared to PC and
0.0007mg/kg bw/day. In 21-day-old pups exposed during gestation and
lactation, testosterone decreased at 0.0007 mg/kg bw/day compared
to PC and 4.0 mg/kg bw/day. Testosterone in pubertal male offspring
decreased at 0.0004 mg/kg bw/day compared to NC and 0.0007 mg/
kg bw/day. Our results suggest that exposure to tembotrione during
gestation and lactation periods disturb sexual hormone levels both in
female and male rat offspring.
Effects of transplacental and translactational exposure to
tembotrione on sex hormone levels in rat offspring until puberty
Anja Mikolic, Irena Brcic Karaconji, Vedran Micek and Davor Zeljezic
Institute for Medical Research and Occupational Health, Zagreb, Croatia
Biography
Anja Mikolic has graduated at the Faculty of Food Technology and
Biotechnology, University of Zagreb in 2005. She acquired PhD in
Biomedicine and Health Sciences at the Faculty of Pharmacy and
Biochemistry, University of Zagreb in 2015. She is employed at Institute
for Medical Research and Occupational Health, Zagreb, Croatia since
2007. She has published as Co-author of 10 original scientific papers in
the international peer-reviewed journals, participated on national and
international scientific conferences and training courses and she is a
member of few scientific associations.
akatic@imi.hrAnja Mikolic et al., J Org Inorg Chem 2018, Volume 4
DOI: 10.21767/2472-1123-C3-009