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Pain Management 2018
Internal Medicine 2018
International Journal of Anesthesiology & Pain Medicine
ISSN: 2471-982X
Page 64
March 26-28, 2018
Vienna, Austria
JOINT EVENT
7
t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Internal Medicine and Patient Care
&
6
t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Pain Management
Volume 4
K
lebsiella pneumonia
is one of the most common bacteria
causing pneumonia, especially in hospitals. The incidence of
carbapenem-resistant klebsiella pneumoniae carbapenemases
(KPC) strains are in increasing manner. Even the multi-drug
resistance strains evolution increasing, the virulence of them
are under question. In this study, we are aiming to study the
relationship between KPC strains and virulence in pneumonia
infection. Three KPC clinical isolates and one multi-drug resistant
non-KPC strain were included in this study. KPC strains were
confirmed to contain plasmid having bla KPC gene. When ICR
mice infected with different KPC strains, mice were apparently
healthy and didn’t showanymortality during 21 days ofmonitoring
while Non-KPC strain showed ~90% mortality within two days of
infection in all inocula used. Infecting neutropenic mice (induced
by cyclophosphamide 200 mg/Kg and cortisone acetate 250 mg/
Kg at day -2, +3, and +8 relative to infection) with KPC strains
using higher inocula were carried out to confirm lower virulence
of KPC strains, in which the mice start to die after day +3 relative
to infection. Screening of all the strains for eight virulent capsular
genes which associated mostly with pathogenesis and invasion
was done. It showed that virulent Non-KPC strain has a highly
virulent gene
wcaG
which encodes capsular fucose synthesis,
and enhance the ability of the bacteria to evade phagocytosis by
macrophages. While the KPC strains didn’t include any of these
capsular virulent genes.
Klebsiella
isolates having KPC plasmid
process low virulence than non-KPC strains in pneumonia
infection.
Biography
Eman G Youssef is a PhD student at Beni-Suef University. She is a Teaching
Assistant at Department of Biotechnology, Faculty of Postgraduate Stud-
ies for Advanced Sciences, Beni-Suef University. She performed her PhD
research at Harbour UCLA, CA as a non-degree program for one year fund-
ed by Fulbright. She is interested in discovering new vaccines and to study
about the microbial pathogenesis and immune responses.
emangouda@psas.bsu.edu.egStudy correlation between KPC clinical isolates and virulence
Eman G Youssef
1, 2
, Sameh Soliman
1
and
Ashraf S Ibrahim
1, 3
1
Los Angeles Biomedical Research Institute, Harbor-UCLA Medical Center Campus, USA
2
Beni-Suef University, Egypt
3
David Geffen School of medicine at UCLA, USA
Eman G Youssef et al., Int J Anesth Pain Med 2018, Volume 4
DOI: 10.21767/2471-982X-C1-003