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Journal of Clinical Immunology and Allergy
ISSN: 2471-304X
16
th
EuroSciCon Conference on
Immunology
M a r c h 1 1 - 1 2 , 2 0 1 9
Am s t e r d a m , N e t h e r l a n d s
Immunology 2019
Marjan Jalali Mehr et al., J Clin Immunol Allergy 2019, Volume:5
DOI: 10.21767/2471-304X-C1-009
Objective:
Tuberculosis has long been recognized as a zoonotic disease
and is one of the most important threatening diseases of death yet. Rapid
identification of the species is too important for fast and correct treatment.
Therefore molecular identification is suitable method for this purpose. The
aim of this study was identification of
Mycobacterium
isolates by RD-Typing
technique in tuberculosis patients in Khorasan Razavi province of Iran.
Materials & Methods:
PCR 16S rRNA and IS6110 were used for one hundred
isolates followed by RD-Typing method (RD1, RD4, RD9 and RD12) were
done to differentiate the complex members. PCR-RFLP of oxy-R gene
performed to confirm differentiation between
Mycobacterium tuberculosis
and
Mycobacterium bovis
.
Results:
PCR 16S rRNA and IS6110 amplified 543 and 245 bp which identified
that all isolates are belonging to the genus
Mycobacterium
and
Mycobacterium
tuberculosis
complex (MTBC) respectively. RD-Typing and PCR-RFLP revealed
that, all isolates were detected
Mycobacterium tuberculosis
and none of them
were
Mycobacterium bovis
or other species of the MTBC.
Conclusion:
Our data suggest a low contribution of
Mycobacterium bovis
between human tuberculosis in the Khorasan Razavi province. This is either due
to the widespread use of pasteurized milk and non-use of milk contaminated
with
Mycobacterium bovis
or due to applying of control and eradication scheme
for Bovine tuberculosis in the whole country.
Biography
Marjan Jalalimehr 40 years old is graduated from Azad
University of Iranmajoring in Master of Microbiology . My thesis
was in the field of Molecular Identification of Mycobacterium
In Department Razi Vaccine & Serum Research Institute.
In the section of Tuberculin and passed an experimental period
working on this . I have continued my study and work on some
related techniques in the field of Microbacterium and I am still
involved in this study in Razi institute.
mjjalalimehr@yahoo.comMolecular identification of
Mycobacterium tuberculosis
complex isolates in smear positive clinical samples by RD-Typing
Marjan Jalali Mehr
1
, Nader Mosavari
2
and Saman Soleimanpour
3
1
Islamic Azad University of Saveh, Iran
2
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension
Organization (AREEO), Iran
3
Mashhad University of Medical Sciences, Iran