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Journal of Clinical Immunology and Allergy
ISSN: 2471-304X
16
th
EuroSciCon Conference on
Immunology
M a r c h 1 1 - 1 2 , 2 0 1 9
Am s t e r d a m , N e t h e r l a n d s
Immunology 2019
Ceren Ciraci et al., J Clin Immunol Allergy 2019, Volume:5
DOI: 10.21767/2471-304X-C1-009
T
he NLR family is a relatively newly discovered family of Pattern recognition
receptors whose functions have been examined almost exclusively in
innate immunity. There are 22 known members of the NLR family in humans,
four of which form a multiprotein complex called the inflammasome complex.
Besides inflammasome complex formation, the NLR family members have
been demonstrated to have associations with several diseases such as
artherosclerosis, type II diabetes, obesity, Alzheimer’s disease, gout and
bacterial, viral and parasite infections. One of the members of the NLR family,
NOD-like Receptor 11 (NLRP11) is expressed only in primates; however, its
cellular functions as well as the specific stimulant(s) that activate it are largely
unknown. To examine whether NLRP11 forms an inflammasome complex
and whether it has regulatory roles in shaping adaptive immune responses,
we investigated its potential interactors including ASC and Caspase-1 by co-
IP and determined T cells responses; respectively. We also determined both
extracellular and intracellular IL1β production by ELISA and western blotting,
as a maker for canonical inflammasome pathway activation. High expression
of NLRP11 and expression of costimulatory molecules made Daudi cells an
ideal model to use in our experiments. Given that B cells are professional
antigen presenting cells (APC) that interact with T cells, we co-cultured human
CD4+ primary T cells with Daudi cells in vitro. 40% down regulation of NLRP11
by siRNA in co-cultures resulted in the significant reduction of Th1, Th17
responses and an increase in anti-inflammatory response whereas did not
significantly affect Th2 responses when compared with control co-cultures.
In brief, our studies of NLRP11 suggest a role in regulating adaptive immune
responses.
Biography
Ceren Ciraci has completed her PhD from Iowa State University
and Postdoctoral Studies from University of Iowa Inflammation
Program. She is currently serving as a Junior Faculty at Istanbul
Technical University.
ciracic@itu.edu.trDownregulation of NLRP11 alters human T cell responses in co-
cultures with Daudi cells
Ceren Ciraci, İrem Ozel and ilgin Akkaya
Istanbul Technical University, Turkey