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Journal of Clinical Immunology and Allergy
ISSN: 2471-304X
16
th
EuroSciCon Conference on
Immunology
M a r c h 1 1 - 1 2 , 2 0 1 9
Am s t e r d a m , N e t h e r l a n d s
Immunology 2019
W
e used four-color ImmunoSpot® assays, in conjunction with peptide pools that cover the sequence of tyrosinase (Tyr),
MAGE-3, Melan/MART-1, gp100, and NY-ESO-1 to charact erize the melanoma antigen (MA)-specific CD8 cell repertoire in
PBMC of 40 healthy human donors (HD). Tyr triggered IFN-γ-secreting CD8 cells in 33% HD within 24h of antigen stimulation
ex
vivo
. MAGE-3, Melan/MART-1, and gp100 also induced recall responses in 10%, 5%, and 5% of HD, respectively. At this time point,
these CD8 cells did not yet produce GzB. However, they engaged in GzB production 72h after antigen stimulation. By this 72h
time point ex vivo, 58% of the HD responded to at least one, and typically several, of the MA. A closer characterization of the Tyr-
specific CD8 cell repertoire showed it to be of low affinity, and to entail primarily the stem cell-like subpopulation.
przybyla.anna.ump@gmail.comClonally expanded, stem cell-like melanoma-
antigen specific CD8 memory cells can be
detected in healthy humans
Anna Przybyła
1, 2
, Ting Zhang
1
, Ruliang Li
1
, Diana R. Roen
1
,
Andrzej Mackiewicz
2
and Paul V Lehmann
1
Cellular Technology Ltd, USA
Poznan University of Medical Sciences, Poland
J Clin Immunol Allergy 2019, Volume:5
DOI: 10.21767/2471-304X-C1-009