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Journal of Clinical Immunology and Allergy
ISSN: 2471-304X
16
th
EuroSciCon Conference on
Immunology
M a r c h 1 1 - 1 2 , 2 0 1 9
Am s t e r d a m , N e t h e r l a n d s
Immunology 2019
T
he quest for novel therapeutic interventions becomes important due to increase in allergic and inflammatory disorders
worldwide including developing countries like India. Allergic reactions predominantly are mediated by activation and
degranulation of mast cells through cross-linking of their high affinity surface receptors FcεRI on binding of IgE and allergen.
Mast cell activation leads to release of early phase pre-stored mediators and late phase newly synthesized cytokines. We explored
if mast cells can be trained to become tolerant of allergen. RBL-2H3 mast cell line and primary bone marrow mast cells were used
to study mast cell secondary responses to allergens and their modulation. For this study, cells were first sensitized with DNP-BSA
specific IgE and treated with different combinations of DNP-BSA to mimic allergen challenge
in vitro
. Cells were further sensitized
and treated with IgE and DNP-BSA for a secondary challenge. β-hexosaminidase release and cytokine expression was analysed
after each allergen challenge. Surface receptor expression was also analysed. The β-hexosaminidase release was significantly
decreased after secondary challenge. Secondary challenge also resulted in reduced pro-inflammatory cytokine expression at
mRNA level. Further, use of inhibitors revealed that various signalling pathways and histone modifications are involved in such
modulations. Our study revealed the mast cell responses can be modulated in vitro after a primary challenge, to become tolerant
or less responsive to the next allergen challenge.
rangati129@gmail.comMast cells’ responses to allergen challenge and
their modulation
Rangati Varma and Niti Puri
Jawaharlal Nehru University, India
J Clin Immunol Allergy 2019, Volume:5
DOI: 10.21767/2471-304X-C1-009