immunology-2018-keynote

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1 5 t h I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology
Journal of Clinical Immunology and Allergy
ISSN 2471-304X
J u l y 0 5 - 0 7 , 2 0 1 8
V i e n n a , A u s t r i a
Immunology 2018
Page 30
Mortality due to pneumococcal infections remains high worldwide, augmented
by widespread antibiotic resistance in many pneumococcal strains. To
identify protein antigens that may be involved in the development of protective
immunity to S. pneumoniae, a pneumococcal cell wall protein-enriched extract was
screened using 2-D gel electrophoresis and immunoblotting with sera obtained
longitudinally from children attending day-care centers and sera obtained from
mice immunized with the pneumococcal cell wall protein-enriched extract. The
identified proteins that share no- or low- homology to human proteins and that
are conserved among different S. pneumoniae strains were tested for their ability
to elicit protection against S. pneumoniae challenge in animal models. Moreover
the nature of the elicited immune response was studied in mice. S. pneumoniae
proteins PtsA, GtS, Nox, FlaR, FBA, TF and PTSMAN were amplified from TIGR4
strain, cloned, expressed and purified. Mice were immunized three times
subcutaneously with these proteins in the presence of adjuvant and challenged
two weeks later. Nasopharyngeal and lung colonization levels were quantified
48 hrs following bacterial challenge or survival was monitored daily for seven
days following challenge. The cytokine profile elicited by rFBA was determined
by multiplex ELISA. All seven proteins elicited protective immune responses in
mice as determined by reduced nasopharyngeal and lung colonization, prolonged
survival, and the ability of antibodies obtained from immunized mice to ex-
vivo neutralize bacterial pathogenicity in the intraperitoneal challenge model.
Moreover, rFBA elicits Th1/Th2/Th17-type cytokines in mice. Immunization with
immunogenic proteins elicits protective immune responses in mouse challenge
systems and the induction of Th1, Th2 andTh17 type of immune responses. Taken
together several antigenic and immunogenic protein with no or low homology to
human protein are were identified and found to elicit protective immune response
in the mouse model accompanied by Th1, Th2 and Th17 type protective immune
responses.
Biography
Yaffa Mizrachi Nebenzahl has completed her PhD at The
Weizmann Institute, Rehovot Israel and Postdoctoral studies
at NIH, USA and UCLA School of Medicine. She is the Director
of Molecular Microbiology laboratory in the Shraga Segal
Department of Microbiology, Immunology and Genetics at
the Faculty of Health Sciences in Ben Gurion University of the
Negev, Beer Sheva, Israel. She has published more than 65
papers in reputed journals.
Novel Streptococcus pneumoniae protein
antigen vaccine and the nature of the
immune response elicited by them
Yaffa Mizrachi Nebenzah
Ben Gurion University of the Negev, Israel
Yaffa Mizrachi Nebenzah, Insights Allergy Asthma Bronchitis 2018, Volume: 4
DOI: 10.21767/2471-304X-C1-001