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E u r o S c i C o n C o n f e r e n c e o n
Chemistry
2018
Chemistry 2018
Journal of Organic & Inorganic Chemistry
ISSN 2472-1123
F e b r u a r y 1 9 - 2 0 , 2 0 1 8
P a r i s , F r a n c e
Page 18
T
he utility of charged aerosol detection (CAD) for quantitation has received
considerable attention in the pharmaceutical industry. The operating
principle of drying and applying a charge to particles in the eluent stream
which are directly measured at an electrometer provides a repeatable
response when analyzing compounds which lack a UV chromophore. Due to
the molecular chemistry of the morpholine alcohol HCL salt, it was found that
neither traditional HPLC-UV nor GC-FID analysis was capable of detecting
residual amounts present in the drug substance. Further development studies
examining different combinations of mobile phases, organic additives, columns
(C8, C18, and HILIC) with a CAD detector were performed. It was determined
that HCL salt format of a morpholine compound could be successfully analyzed
by an HPLC –CAD method with a HILIC column. The method proved capable of
controlling residual morpholine alcohol residual in a drug substance.
Biography
D Wang has completed his PhD from Florida International Uni-
versity. He is a Senior Scientist at Alkermes, a global biopharma-
ceutical company. He has published more than 12 papers and
presentations in reputed journals and scientific conferences.
david.wang@alkermes.comN-(2-Hydroxyethyl) morpholine HCL salt content by hydrophilic interaction
liquid chromatography (HILIC) with charged aerosol detection (CAD)
D Wang
1
and P glynn
2
1
Alkermes, USA
2
Alkermes, Ireland
D Wang, J Org Inorg Chem 2018, Volume: 4
DOI: 10.21767/2472-1123-C1-002