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E u r o S c i C o n C o n f e r e n c e o n
Chemistry
2018
Chemistry 2018
Journal of Organic & Inorganic Chemistry
ISSN 2472-1123
F e b r u a r y 1 9 - 2 0 , 2 0 1 8
P a r i s , F r a n c e
Page 44
E
pidemiological studies have linked asthma with vitamin D deficiency
(VDD). Asthma, like VDD, is highly prevalent in Ireland. Vitamin D receptor
(
VDR
) gene polymorphisms have been associated with asthma and allergy
susceptibility. The objective of this project is to set up new biochemical
methods in a clinical laboratory for diagnosing and treating asthma, and to
deepen our knowledge of the illness’s pathophysiology. We successfully
completed verification of the total 25-hydroxyvitamin D (25OHD or C
27
H
44
O
2
)
assays on Abbott Architect. New tests for eosinophil cationic protein (ECP),
IL10, IL17a,
VDR
, cathelicidin antimicrobial peptide (CAMP) and for 4 single
nucleotide polymorphisms (SNPs) were employed for diagnostic and research
testing. We found associations between vitamin D levels (C
27
H
44
O
2
) and
airway obstruction in adults’ asthma and body mass index in healthy Irish
adults. A negative association was recorded between 25OHD and IgE levels in
paediatric patients. In general we observed no significant benefit of vitamin D
supplementation in asthmatics. However improvement in asthma control was
noticeable in some patients with specific genotypes. We showed an association
of TaqI and ApaI polymorphisms of the
VDR
gene with a susceptibility to
asthma in Irish patients. Also, we demonstrated that paediatric patients with
TC for TaqI, and CC and CT genotypes for ApaI have a significantly low level
of IL-10 and increased white blood cells (neutrophils in particular), and that
they were associated with poor asthma control. Vitamin D’s role in respiratory
disorders has not yet been fully investigated. Research is still at an early stage,
but our preliminary results seem encouraging. Further and more extensive
studies, using a larger sample, will be necessary to confirm our findings, to
examine links between vitamin D and
VDR
gene polymorphisms in specific
asthma phenotypes, and to investigate the possibility of using
VDR
SNPs as
biomarkers for susceptibility to asthma.
Biography
Katrina Hutchinson has a MD fromMoscow State Medical Uni-
versity and she is currently completing her PhD in the School of
Chemistry in the National University of Ireland, Galway. She has
published several articles based on her research. Since 2000
she has been the Head of the Clinical Chemistry, Immunology,
Serology and Molecular Biology departments at Eurofins-Biom-
nis, Ireland. She was short-listed by Enterprise Ireland for the
Irish Scientist of the Year in 2013, and she has been an active
participant in national and international conferences relating to
Clinical Chemistry, Endocrinology and Immunology. .
Katrina.hutchinson@eurofins-biomnis.ieA source of new diagnostic techniques for asthma
Katrina Hutchinson
1,3
, John Faul
2
, Michael Louw
1
and Yury
Rochev
3
1
Eurofins-Biomnis, Ireland
2
Connolly Hospital Blanchardstown, Ireland
3
National University of Ireland, Galway, Ireland
Katrina Hutchinson, J Org Inorg Chem 2018, Volume: 4
DOI: 10.21767/2472-1123-C1-002