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allied
academies
August 17-18, 2017 | Toronto, Canada
ANNUAL BIOTECHNOLOGY CONGRESS
Ann Biol Sci, 2017
ISSN: 2348-1927
I
n this study, we investigated the antimicrobial properties
of pure zinc oxide nanoparticles (ZnO NPs), Cu NPs
and Fe
2
O
3
NPs, against two plant pathogenic fungi:
Fusarium oxysporum
,
Alternaria solani
and one bacterial
strain:
Clavibacter michiganensis,
which are the main
microorganisms responsible for severe diseases of a large
number of agricultural crops. Metal nanoparticles were
applied at various concentrations (0, 250, 500 and 1000
mg L-1) to determine their
in vitro
antimicrobial activity.
We synthesized ZnO NPs at room temperature by using a
mechanically assisted metathesis reaction that permitted
the formation of spherical NPs with mean sizes of around
20-30 nm. NPs characterization was accomplished by X-ray
diffraction; particles size and shape were determined by
TEM. We compared the effect of engineered NPs against
commercial Cu and Fe
2
O
3
NPs of similar size and shape.
Antifungal activity of NPs was evaluated on PDA media,
and King´s B medium was used for bacteria. ANOVA and
Tukey multiple range tests were employed to analyse data.
Cu NPs showed the greatest antimicrobial activity against
both fungal strains, followed for ZnO NPs. In contrast, ZnO
produced maximum growth inhibition against the bacteria
C.
michiganensis
. On the other hand, Fe
2
O
3
NPs did not exhibit
antimicrobial activity with these phytopathogenic strains.
Based on these results, it is viable that tested ZnO and Cu
NPs could be used in programs of sustainable agriculture,
since they are required in minute quantities by comparison
to conventional pesticides.
Speaker Biography
Vera-Reyes I has completed her PhD from the Centro de Investigación en Estudios
Avanzados del Instituto Politecnico Nacional, Mexico D F. She is a CONACYT Research
Fellow.
e:
ileana.vera@ciqa.edu.mxAntagonistic effect of metallic nanoparticles on phyto-pathogenic fungi and bacteria
Vera-Reyes I
1
,
3
, Lira-Saldivar R H
1
, Esparza-Arredondo IJE
2
, Méndez-Argüello B
1
and
García-Cerda L A
1
1
Centro de Investigación en Química Aplicada, Mexico
2
Universidad Autónoma Agraria Antonio Narro, Mexico
3
CONACYT-CIQA, Mexico
Vera-Reyes I et al., Ann Biol Sci, 2017, 5:3
DOI: 10.21767/2348-1927-C1-002