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alliedacademies
August 17-18, 2017 | Toronto, Canada
ANNUAL BIOTECHNOLOGY CONGRESS
Ann Biol Sci, 2017
ISSN: 2348-1927
Brucellosis is an infectious disease that affects practically
all species of mammals, including humans, and is a major
zoonosis worldwide. Brucella sp. are facultative intracellular
pathogens that have the ability to survive and multiply in
phagocytic and non-phagocytic cells such as trophoblast
and epithelial cells. Among the six recognized species of the
genus Brucella, Brucella melitensis is the main etiological
agent involved in goat brucellosis and is also the most
pathogenic for human. It causes significant losses in livestock
production as a result of abortions, metritis, infertility, and
birth of weak animals. Outer membrane proteins (OMPs) are
exposed on the bacterial surface and are in contact with cells
and effectors of the host immune response, whereby they
could be important virulence factors of Brucella species. To
evaluate this hypothesis, the gene encoding for the major
outer membrane protein Omp31 was amplified, cloned into
pUC18 plasmid, and inactivated by inserting a kanamycin
cassette, rendering pLVM31 plasmid which was transformed
into B. melitensis wild-type strain to obtain LVM31 mutant
strain. The outer membrane (OM) properties of the mutant
strain were compared with B. melitensis Bm133 wild-type
and B. melitensis Rev1 vaccine strains, in assessing its
susceptibility to polymyxin B, sodium deoxycholate, and
nonimmune serum. The mutant strain was assessed in vitro
with survival assays in murine macrophages J774.A1 and
HeLa cells. Our results demonstrate that LVM31 mutant is
more susceptible to polymyxin B, sodium deoxycholate,
and nonimmune serum than control strains. Moreover,
Omp31 mutation caused a decrease in the internalization
and a significant decrease in the intracellular survival
compared with the reference strains in both cell lines.
These results allow us to conclude that Omp31 is important
for maintaining OM integrity, but also it is necessary for
bacterial internalization, establishment and development of
an optimal replication niche, and essential for survival and
intracellular multiplication.
Speaker Biography
Lázaro Verdiguel-Fernández has completed his Master’s degree from National
Autonomous University of Mexico. Currently, he is a PhD student. He is Professor
of Veterinary Immunology and Applied Molecular Microbiology and directed five
undergraduate thesis. He has published one paper in Archives of Microbiology Journal.
He has participated in the International Brucellosis Research Conference including the
“69th Annual Brucellosis Research Meeting”, New Delhi, India, 2016. He is a member
of the Biotechnology Committee of the National Technical Advisory Council on Animal
Health of Mexico.
e:
Omp31 plays an important role on outer membrane properties and intracellular survival of Brucella
melitensis in murine macrophages and HeLa cells
Lázaro Verdiguel-Fernández
Universidad Nacional Autónoma de México, Mexico
Lázaro Verdiguel-Fernández, Ann Biol Sci, 2017, 5:3
DOI: 10.21767/2348-1927-C1-002