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August 17-18, 2017 | Toronto, Canada
ANNUAL BIOTECHNOLOGY CONGRESS
Ann Biol Sci, 2017
ISSN: 2348-1927
E
arly weaning of pigs can lead to low feed intake resulting
in a lag in growth, performance and intestine infection.
Epidermal growth factor (EGF), the most abundant growth
factor in milk, increased weaned pig body weight gain
and feed efficiency in our previous work. It is believed
that intestinal microbiota plays an important role in pig
growth but data is limited on the impact of feed additives
on intestinal microbiota. The objective of the study is to
investigate, if the positive influence on weight gain, and
intestinal health with dietary EGF supplementation is related
to differences in intestinal microbiota.
Pichia pastoris
were
engineered to secrete porcine EGF using codon-optimized
sequence. To examine the efficacy of EGF, an animal trial was
performed using 72 pigs (2 equal blocks of 36 pigs with 3
barrows and 3 gilts/pen). The animals were assigned to one
of two dietary treatments at weaning (20±2 d of age; n=6
pens/treatment) balancing across treatment for litter, gender
and initial BW. Supernatant with EGF at 120 µg/kg BW/d
and without EGF (control) was added to the feed for 21 d,
followed by a common diet for 7 d. Animal performance was
monitored on a weekly basis and ileal digesta samples were
collected for microbiome analysis after 21 d of treatment.
Pigs fed diets containing EGF fermentation supernatant
had a greater (P=0.01) overall daily gain which is consistent
to our previous finding. No difference in alpha-diversity
(Chao1, Shanon, and Simpson indices) and beta-diversity
(weighted and unweighted UniFrac distances) of ileal digesta
microbiota between EGF supplemented and control pigs
were observed. The relative abundances of bacterial taxa
did not differ among treatment groups at the phylum level;
however, the abundances of
Corynebacterium
(0.0 vs 0.9%),
Blautia
(0.003 vs 0.26%), and
Coprococcus
(0.0 vs 0.05%)
genera and
Rumminococcaceae
family (0.001 vs 0.08%) were
decreased (P<0.05) in EGF group compared to control, which
might positively influence intestinal health.
Speaker Biography
Nadeem Akhtar has completed his PhD in Biotechnology from Thapar University, India
and is currently is a Post-doctoral fellow in the Department of Animal Biosciences,
University of Guelph, Canada. He has published more than 15 papers in reputed
journals and filed a patent in India. He has also been serving as a Reviewer for few
reputed journals such as
Environmental Progress & Sustainable Energy,
Journal of
Taiwan Institute of Chemical Engineers, Bioinfo Publications
etc.
e:
akhtarn@uoguelph.caThe impact of epidermal growth factor (EGF) supernatant on pig performance and ileal microbiota
Nadeem Akhtar
1
, Crystal Levesque
2
, Evanna Huynh
1
, Carrie Walk
3
, Pete Wilcock
3
, Zhengxiao Zhang
4
, Paul Dyce
4
, Cornelis F MDe Lange
1
, Ehsan Khafipour
4
,
and Julang Li
1
1
University of Guelph, Canada
2
South Dakota State University, USA
3
AB Vista, United Kingdom
4
University of Manitoba, Canada
Nadeem Akhtar et al., Ann Biol Sci, 2017, 5:3
DOI: 10.21767/2348-1927-C1-002