5 / 23
Page 45
Notes:
allied
academies
August 17-18, 2017 | Toronto, Canada
ANNUAL BIOTECHNOLOGY CONGRESS
Ann Biol Sci, 2017
ISSN: 2348-1927
A
cute myocardial infarction (AMI) occurs when a coronary
artery is clogged, in 80% of the cases, by coronary
atherosclerosis with superimposed luminal thrombus.
This occlusion leaves the downstream zone of the heart
without blood supply. As a result, the papillary muscles are
separated, what leads to regurgitation, contributing to the
overload of the heart. Cardiac muscle engineering aims at
providing functional myocardium to repair diseased hearts
and model cardiac development, physiology, and disease
in vitro
. The objective of the present
in vitro
study was to
prepare, characterize and assess polycaprolactone (PCL)/
fibrin glue (FG)/multi wall carbon nanotube (MWCNTs)
nanocomposite scaffolds, to guide regeneration of
myocardial tissue. For this purpose, two different weight
ratio of multi wall carbon nanotubes (1 and 0.5 % wt) were
added to the pure PCL polymeric scaffold by solvent casting
process. The nanocomposite scaffolds were coated by fibrin
glue and the solvent was removed from the structure by
freeze drying technique. Characterization technique such as
Scanning Electron Microscopy (SEM), Transmission Electron
Microscopy (TEM), FT Infrared Spectroscopy (FTIR) and X-ray
Diffraction (XRD) were performed. Tensile tests were carried
out for evaluating the mechanical properties. To evaluate
the cytotoxicity of scaffolds, MTT assay were performed with
mouse myoblast cells in 1, 4 and 7 days. Biodegradability,
electrical conductivity as well as contact angle and wettability
of the nanocomposite scaffolds were investigated. Carbon
nanotubes have crystalline structure, electrical conductivity,
and particle size was in the range of 20-30 nanometer. Both
coated and uncoated nanocomposite scaffolds showed
appropriate cell response during the period of specified
times. Meanwhile, the adhesion of the cells was more for
coated nanocomposite scaffolds. Addition of MWCNTs to
the pure PCL polymeric scaffolds significantly raised the
electrical conductivity. MWCNTs have a good adhesion with
fibrin glue in coated samples. In the presence of carbon
nanotubes, the elastic modulus of the nanocomposite
scaffolds compare to the pure PCL polymeric scaffolds were
increased. In vitro degradation assessment exhibited that
samples had significant weight loss after two months and
the degradation of the samples were increased not only
by adding MWCNTs but also by coating the samples with
fibrin glue. In the presence of fibrin glue, nanocomposite
scaffolds became hydrophilic and contact angle was
decreased. It was concluded that bioactive, degradable
and electrical conductive nanocomposite scaffolds made of
polycaprolactone/fibrin glue/multi wall carbon nanotubes
could be used as an appropriate construct for reconstruction
and restoration of damaged myocardial tissue..
Speaker Biography
Sharareh Ghaziof has completed her Master’s degree in Biomedical-Tissue Engineering
from Islamic Azad University, Najafabad Branch, Najafabad, Iran. She has developed
her passion for academic research and experiences in tissue engineering, drug delivery
and related topics at University of Isfahan and Isfahan University of Medical Science
(Central laboratory, School of Medicine).
e:
sh_gh_256@yahoo.comRenovation of the injured myocardial tissue with new conductive, biodegradable, and non-cytotoxic
polymer matrix coated with fibrin glue nanocomposite scaffold
Sharareh Ghaziof
1
and
Mehdi Mehdikhani-Nahrkhalaji
2
1
Islamic Azad University, Iran
2
University of Isfahan, Iran
Sharareh Ghaziof et al., Ann Biol Sci, 2017, 5:3
DOI: 10.21767/2348-1927-C1-003