2 / 23
Page 42
Notes:
allied
academies
August 17-18, 2017 | Toronto, Canada
ANNUAL BIOTECHNOLOGY CONGRESS
Ann Biol Sci, 2017
ISSN: 2348-1927
A
ntimicrobial
peptides(AMPs) are regarded as host
defense peptides which processes bactericidal, and they
havealsobeen reported tohave immunomodulatory function
in many tissues. However, their role in the mammalian ovary
is unknown. We have previously found that beta defensin 2
(BD2) and beta defensin 3 (BD3) are expressed in granulosa
cells and cumulus cells during porcine ovarian follicular
development and cumulus-oocyte-complex maturation.
We hypothesized that these antimicrobial peptides are
involved in the regulation of follicular development in
ovaries. Granulosa cells were isolated from small (1-3 mm in
diameter) and large (4-8mm in diameter) porcine follicles and
cultured in the absence and presence of 1, 10, and 50 µg/ml
of BD2, and BD3. After 24 hours of treatment, cell numbers
were counted using an automated cell counter. It was found
that while BD2 appears to have no effect, BD3 stimulated
granulosa cell proliferation in a dose dependent manner (p <
0.05). This effect is also dependent on the stage of follicular
development, as it is effective on granulosa cell from small
but not large follicles. In addition, transwell cell migration
assay revealed that in the presence of BD3 (10 µg/ml), a
2.5 fold increase in cell migration was achieved. To further
study the potential pathway involved in BD3 induced cell
proliferation; western blots were performed to determine
the ratio of phosphorylated- and non-phosphorylated-
ERK1/2. It was found that BD3 significantly increased the
phosphorylated- ERK1/2 ratio. Moreover, U0126, the specific
ERK1/2 phosphorylation inhibitor, suppressed BD3 induced
ERK1/2 phosphorylation and proliferation, suggesting that
BD3 may stimulate granulosa cell proliferation via activating
the MAPK pathway. Our data suggests that antimicrobial
peptides may play a physiological role, in addition to being
the traditionally recognized immune-defense mechanism, in
regulating follicular development in the mammalian ovary.
Speaker Biography
Liu Canying has completed her PhD at the age of 28 years from Huazhong Agricultural
University College of Veterinary Medicine. She has just graduated and been the lecturer
of Foshan University Department of Veterinary Medicine for about one year. She has
published 4 papers in famous journals. She mainly performed research projects about
the comparative genomic and pathogenicity analyses of bacteria, immunogenicity
analyse of outer membrane proteins of bacteria.
e:
canyingliu@gmail.comBeta defensin 3 enhances ovarian granulosa cell proliferation via MAPK pathway
Canying Liu
1, 2
, Bo Pan
2
, Bingyun Wang
1
and
Julang Li
2
1
Foshan University, China
2
University of Guelph, Canada
Canying Liu et al., Ann Biol Sci, 2017, 5:3
DOI: 10.21767/2348-1927-C1-003