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August 17-18, 2017 | Toronto, Canada
ANNUAL BIOTECHNOLOGY CONGRESS
Ann Biol Sci, 2017
ISSN: 2348-1927
T
he Epstein-Barr virus (EBV) is a herpes virus infecting more
than 90% of the human population. The tropism of EBV
for B lymphocytes is evidenced in its association with many
lymphoproliferative disorders. Different types of EBV (EBV-1
and EBV-2), classified on the basis of EBNA-2 genotyping, have
been reported in benign and malignant pathologies, but there
is almost no information about their frequency in the Pakistani
population. The aim of this study was to determine the
frequency and distribution of EBNA-2-based EBV genotypes in
lymphomapatients. GenomicDNAwas extracted fromformalin-
fixed paraffin embedded (FFPE) tissue samples obtained from
73 EBV-DNA-positive lymphoma patients. The β-globin gene
was amplified to assess the presence and quality of cellular
DNA from all samples. EBER-1 DNA was detected by PCR to
confirm EBV presence in tissue samples. EBNA-1 mRNA relative
quantification by quantitative PCR substantiated EBNA-1mRNA
overexpression in 43.8% of EBV-positive cases in comparison to
an EBV-positive control cell line. EBNA-2 genotyping was done
by nestedpolymerase chain reaction (PCR). Among the samples,
EBV-1 was present in 90.7% and EBV-2 in 9.3%. These results
show that EBV-1 is the most prevalent type in the lymphoma
population of Pakistan, similar to reports from other countries.
This definition of EBV epidemiology in Pakistani lymphoma
patients represents an important first step in using EBV for
prognosis and monitoring treatment response in patients.
Speaker Biography
Sadia Salahuddin is PhD scholar at Gomal University. She worked at Cornell University
and University of North Carolina as research scholar for four years. She has authored
number of good quality research articles in reputed journals and has been serving as
an editorial board member of reputed journal.
e:
sadia.salahuddin1@gmail.comPrevalence of Epstein–Barr virus genotypes in Pakistani lymphoma patients
Sadia Salahuddin
1, 2, 3
1
Cornell University, USA
2
The University of North Carolina, USA
3
Gomal University, Pakistan
Sadia Salahuddin, Ann Biol Sci, 2017, 5:3
DOI: 10.21767/2348-1927-C1-003