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Volume 3, Issue 2 Insights in Analytical Electrochemistry
ISSN: 2470-9867 Analytical Chemistry-Formulation 2017
August 28-30, 2017
Page 44
8 th Annual Congress on
&
14th International Conference and Exhibition on
August 28-30, 2017 Brussels, Belgium
Analytical and Bioanalytical Techniques
Pharmaceutical Formulations
Mass spectrometry: A complementary tool to ELISA for allergen detection
M Planque1,2, A C Huet1, T Arnould2, A Lamote1, M Dieu2, P Renard2 and N Gillard1
1CER Groupe, Health Department, Belgium
2University of Namur, Belgium
Food allergies rise increasingly over the last decades. To protect themselves, food customers must exclude the allergenic
food. Unfortunately, unintentional contaminations in finish products are still possible due to cross-contamination during
food production, and food storage. To help producers, food laboratories developed methods for the protection of allergic
customers. Most contaminations of food products by allergens are determined by enzyme-linked immunosorbent assay
(ELISA). However, high-baked allergens in food products are sometimes hardly detected by ELISA. Ultra-high-performance
liquid chromatography coupled to tandem mass spectrometry recently developed allows a highly specific and sensitive
detection of processed allergens in food products. The establishment of a UHPLC-MS/MS method is expensive compared
to ELISA method. Both methods present advantages and disadvantages, but, they are complementary. The guideline SMPR
2016.002, published in 2016, is dedicated for UHPLC-MS/MS methods. Food products selected in this guideline will be
analyzed by ELISA and UHPLC-MS/MS and compared. This study will present the complementarity of UHPLC-MS/MS and
ELISA method for a better use and comprehension of methods for the detection of allergens.
Biography
M Planque holds a Master’s degree in Chemistry. She started her PhD in 2014 at CER Groupe (Health Department) and at the University of Namur in Belgium. She
is currently working on the sensitive detection of allergens by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry.
M Planque et al., Insights in Analytical Electrochemistry, 3:2
DOI: 10.21767/2470-9867-C1-002