Previous Page  22 / 39 Next Page
Information
Show Menu
Previous Page 22 / 39 Next Page
Page Background

Notes:

Volume 3, Issue 2

Insights in Analytical Electrochemistry

ISSN: 2470-9867

Analytical Chemistry-Formulation 2017

August 28-30, 2017

Page 41

8

th

Annual Congress on

&

14

th

International Conference and Exhibition on

August 28-30, 2017 Brussels, Belgium

Analytical and Bioanalytical Techniques

Pharmaceutical Formulations

Effect of laser-induced shockwave on molecules and particles in solution

Nobuyuki Ichinose

Kyoto Institute of Technology, Japan

F

ocusing of a nanosecond laser pulse (≈200

µ

J) into aqueous solutions with an objective lens generates a high temperature

plasma by dielectric breakdown, which induces generation of shockwave. Propagation of the shockwave with a high pressure

causes linear and non-linear effects on the solute or dispersed particles. Time-resolved fluorescence spectroscopic observation

under microscope has revealed that the shockwave affects local concentration of solutes due to a sub-mm movement of

the molecules/particles with a near-sonic velocity in water. Combination of a ≈100

µ

m capillary to confine the shockwave

propagation into one-dimension and a collagen gel to control the holding and releasing of the loaded molecules/particles made

their movement give a spot as if they were brought by a laminar flow. The distance travelled of a few tens to hundreds

µ

m by

the fluorescent-labeled proteins, DNAs, and polysaccharides or CdSe nanoparticles was found to be molecular type- and size-

dependent. This technique (laser-induced shockwave chromatography) can avoid unwanted adhesion onto the solid stationary

phase and will be applicable to prompt analysis to study aggregation/polymerization phenomena of biomolecules.

Biography

Nobuyuki Ichinose received his PhD from Osaka Prefecture University, Japan. He is the professor of Kyoto Institute of Technology, Japan. He has over 60

publications in various fields in chemistry.

ichinose@kit.ac.jp

Nobuyuki Ichinose, Insights in Analytical Electrochemistry, 3:2

DOI: 10.21767/2470-9867-C1-002