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Volume 4
Nano Research & Applications
ISSN: 2471-9838
JOINT EVENT
October 04-05, 2018 Moscow, Russia
&
2
nd
Edition of International Conference on
26
th
International Conference on
Advanced Nanotechnology
Materials Technology and Manufacturing Innovations
Advanced Nanotechnology 2018
& Materials-Manufacturing 2018
October 04-05, 2018
Page 58
Anna V Bychkova
N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Russia
Anna V Bychkova et al., Nano Res Appl 2018, Volume 4
DOI: 10.21767/2471-9838-C5-020
Stable plasma protein coatings on magnetite nanoparticles for biomedical applications
Mariia V. Lopukhova, Alexander I. Shalupov, Luybov A. Wasserman, Pavel G. Pronkin, Alexander L. Kovarski
and
Mark A. Rosenfeld
N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Russia
P
roteinsarepromisingmaterialsforcreationofcoatingsonmagneticnanoparticles(MNPs)duetotheirbiocompatibility,
preventionofMNPs agglomerationandchemical reactions inbiological liquids [1].Magnetically targetednanosystems
with protein coatings are considered to be applicable in different areas of biology and medicine including theranostics
and biosensing techniques. Proteins represent extremely susceptible targets for oxidants. The protection mechanisms
in preventing oxidative damages for proteins within cells are mainly related to a large variety of antioxidant enzymatic
systems. In contrast, plasma proteins are scarcely protected by these systems but the highly site-specific oxidation was
convincingly demonstrated for some proteins, indicating that protein structure could be adapted to oxidation [2]. The
mechanism providing plasma protein functioning in the conditions of generating reactive oxygen species (ROS) is a
base for to the development of free radical approach to immobilizing of protein on magnetic nanoparticles (MNPs)
in dispersions. Adsorption of a group of blood proteins including serum albumin and immunoglobulin G on MNPs
and stability of the coatings was studied with the help of dynamic light scattering (DLS), UV/Vis spectrophotometry,
differential scanning calorimetry (DSC), spin label technique [3], ferromagnetic resonance (FMR) [4], and the method
of spectral-fluorescent probes [5]. The novel approach lead to the formation of stable cross-linked functional coatings on
magnetite (Fe
3
O
4
) MNPs assembled from protein molecules. The free radical linking of thrombin and immunoglobulin
G on the surface of nanoparticles has been shown to almost completely keep native properties of the protein molecules
as potential therapeutic products and biovectors. The reported study aimed at obtaining multifunctional coating on
magnetic nanoparticles was funded by RFBR and Moscow city Government according to the research project № 15-33-
70019 «mol_а_mos», and by the Russian Science Foundation project No. 18-73-00350; protein study on the surface of
MNPs was funded by RFBR, according to the research project No. 16-34-60244 mol_а_dk; spectral-fluorescent probes
applications were developed according to the research RFBR project No. 16-03-00735 a. The research was carried out
within the framework of budget financing under the government task (themes 0084-2014-0001, State registration No
01201253311, and 0084-2014-0005, State registration No 01201253307).
Recent Publications
1. Bychkova, A.V.; Sorokina, O.N.; Rosenfeld, M.A.; Kovarski, A.L. Multifunctional biocompatible coatings on
magnetic nanoparticles. Russian Chemical Reviews. 2012. Vol. 81 (11). PP. 1026-1050.
2. Rosenfeld, M.A.; Vasilyeva, A.D.; Yurina, L.V.; Bychkova, A.V. Oxidation of proteins: is it a programmed process?
Free Radical Research, 2018, Vol. 52, No. 1. PP. 14–38.
3. Sorokina, O.N.; Bychkova, A.V.; Shapiro, A.B.; Kovarskii, A.L.; Tikhonov, A.P. The application of the spin label
method for studying the adsorption of macromolecules on magnetic nanoparticles. Russian Journal of Physical